Characterize the Growth and Metabolism of <i>Acidithiobacillus ferrooxidans</i> under Electroautotrophic and Chemoautotrophic Conditions

doi:10.3390/microorganisms12030590

CORC > 深海科学与工程研究所 > 中国科学院深海科学与工程研究所 > 深海科学研究部 > 地外海洋系统研究室

	*Characterize the Growth and Metabolism of Acidithiobacillus ferrooxidans* under Electroautotrophic and Chemoautotrophic Conditions**
	Wang, Quansheng 1,2; Long, Haijun 1,2; Wang, Huiqi 2; Lau Vetter, Maggie C. Y.2
刊名	MICROORGANISMS
	2024-03-01
卷号	12 期号:3 页码:26
关键词	acidophile Acidithiobacillus ferrooxidans electroautotrophic chemoautotrophic transcriptome metabolome
DOI	10.3390/microorganisms12030590
英文摘要	Acidophiles are capable of surviving in extreme environments with low pH. Acidithiobacillus ferrooxidans is a typical acidophilic bacterium that has been extensively studied when grown chemoautotrophically, i.e., when it derives energy from oxidation of Fe2+ or reduced inorganic sulfur compounds (RISCs). Although it is also known to grow with electrons supplied by solid electrodes serving as the sole source of energy, the understanding of its electroautotrophic growth is still limited. This study aimed to compare the growth characteristics of A. ferrooxidans under electroautotrophic (ea) and chemoautotrophic (ca) conditions, with an attempt to elucidate the possible mechanism(s) of extracellular electron flow into the cells. Jarosite was identified by Raman spectroscopy, and it accumulated when A. ferrooxidans used Fe(2+ )as the electron donor, but negligible mineral deposition occurred during electroautotrophic growth. Scanning electron microscopy (SEM) showed that A. ferrooxidans possesses more pili and extracellular polymeric substances (EPSs) under electroautotrophic conditions. A total of 493 differentially expressed genes (DEGs) were identified, with 297 genes being down-regulated and 196 genes being up-regulated in ea versus ca conditions. The genes known to be essential for chemoautotrophic growth showed a decreased expression in the electroautotrophic condition; meanwhile, there was an increased expression of genes related to direct electron transfer across the cell's outer/inner membranes and transmembrane proteins such as pilin and porin. Joint analysis of DEGs and differentially expressed metabolites (DEMs) showed that galactose metabolism is enhanced during electroautotrophic growth, inducing A. ferrooxidans to produce more EPSs, which aids the cells in adhering to the solid electrode during their growth. These results suggested that electroautotrophy and chemoautotrophy of A. ferrooxidans have different extracellular electron uptake (EEU) pathways, and a model of EEU during electroautotrophic growth is proposed. The use of extracellular electrons as the sole energy source triggers A. ferrooxidans to adopt metabolic and subsequently phenotypic modifications.
资助项目	Institute of Deep-sea Science and Engineering, Chinese Academy of Sciences
WOS关键词	EXTRACELLULAR ELECTRON-TRANSFER ; THIOBACILLUS-FERROOXIDANS ; BC(1) COMPLEXES ; OUTER-MEMBRANE ; FE2+ OXIDATION ; FERROUS IRON ; ATCC 23270 ; IV PILI ; BACTERIUM ; CHALCOPYRITE
WOS研究方向	Microbiology
语种	英语
出版者	MDPI
WOS记录号	WOS:001193528200001
资助机构	Institute of Deep-sea Science and Engineering, Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.idsse.ac.cn/handle/183446/10962]
专题	深海科学研究部_地外海洋系统研究室
通讯作者	Lau Vetter, Maggie C. Y.
作者单位	1.Univ Chinese Acad Sci, Beijing 101408, Peoples R China 2.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Lab Extraterr Ocean Syst LEOS, Sanya 572000, Peoples R China
推荐引用方式 GB/T 7714	Wang, Quansheng,Long, Haijun,Wang, Huiqi,et al. Characterize the Growth and Metabolism of Acidithiobacillus ferrooxidans under Electroautotrophic and Chemoautotrophic Conditions[J]. MICROORGANISMS,2024,12(3):26.
APA	Wang, Quansheng,Long, Haijun,Wang, Huiqi,&Lau Vetter, Maggie C. Y..(2024).Characterize the Growth and Metabolism of Acidithiobacillus ferrooxidans under Electroautotrophic and Chemoautotrophic Conditions.MICROORGANISMS,12(3),26.
MLA	Wang, Quansheng,et al."Characterize the Growth and Metabolism of Acidithiobacillus ferrooxidans under Electroautotrophic and Chemoautotrophic Conditions".MICROORGANISMS 12.3(2024):26.