Functional analyses of TRAF6 gene in Argopecten scallops

doi:10.1016/j.fsi.2024.109443

	Functional analyses of TRAF6 gene in Argopecten scallops
	Zhang, Xiaotong 1; Wang, Xia 1; Chen, Jieyu 2; Chen, Min 3; Lu, Xia 3; Ning, Junhao 3; Liu, Haijun 4; Liu, Guilong 4; Xu, Xin 4; Qu, Xiaoxu 1
刊名	FISH & SHELLFISH IMMUNOLOGY
	2024-04-01
卷号	147 页码:9
关键词	Argopecten irradians (Air) Argopecten purpuratus (Apu) Tumor necrosis factor (TNF) TNF receptor-associated factor (TRAF) Subcellular localization RNA interference (RNAi)
ISSN号	1050-4648
DOI	10.1016/j.fsi.2024.109443
通讯作者	Wang, Chunde(chundewang2007@163.com) ; Liu, Bo(liubomusic@126.com)
英文摘要	The tumor necrosis factor (TNF) receptor-associated factor (TRAF) family has been reported to be involved in many immune pathways. In a previous study, we identified 5 TRAF genes, including TRAF2, 3, 4, 6, and 7, in the bay scallop (Argopecten irradians, Air) and the Peruvian scallop (Argopecten purpuratus, Apu). Since TRAF6 is a key molecular link in the TNF superfamily, we conducted a series of studies targeting the TRAF6 gene in the Air and Apu scallops as well as their hybrid progeny, Aip (Air 9 x Apu d) and Api (Apu 9 x Air d). Subcellular localization assay showed that the Air-, Aip-, and Api-TRAF6 were widely distributed in the cytoplasm of the human embryonic kidney cell line (HEK293T). Additionally, dual-luciferase reporter assay revealed that among TRAF3, TRAF4, and TRAF6, only the overexpression of TRAF6 significantly activated NF-kappa B activity in the HEK293T cells in a dose-dependent manner. These results suggest a crucial role of TRAF6 in the immune response in Argopecten scallops. To investigate the specific immune mechanism of TRAF6 in Argopecten scallops, we conducted TRAF6 knockdown using RNA interference. Transcriptomic analyses of the TRAF6 RNAi and control groups identified 1194, 2403, and 1099 differentially expressed genes (DEGs) in the Air, Aip, and Api scallops, respectively. KEGG enrichment analyses revealed that these DEGs were primarily enriched in transport and catabolism, amino acid metabolism, peroxisome, lysosome, and phagosome pathways. Expression profiles of 28 key DEGs were confirmed by qRT-PCR assays. The results of this study may provide insights into the immune mechanisms of TRAF in Argopecten scallops and ultimately benefit scallop breeding.
WOS关键词	NF-KAPPA-B ; BAY SCALLOP ; MOLECULAR CHARACTERIZATION ; INBREEDING DEPRESSION ; TRAITS
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
WOS记录号	WOS:001188218600001
资助机构	National Natural Science Foundation of China ; Earmarked Fund for Agriculture Seed Improvement Project of Shandong Province ; Scientific and Technological Project of Yantai, Shandong Province ; Earmarked Fund for Shandong Modern Agro-Industry Technology Research System ; Science and Technology Innovation Capacity Improvement Project of Shandong Province ; Natural Science Foundation of Shandong Province
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/34925]
专题	中国科学院烟台海岸带研究所
通讯作者	Wang, Chunde; Liu, Bo
作者单位	1.Qingdao Agr Univ, Coll Marine Sci & Engn, Qingdao 266109, Shandong, Peoples R China 2.Qingdao 9 High Sch, Qingdao 266426, Shandong, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresour, Yantai 264003, Shandong, Peoples R China 4.Yantai Spring Sea AquaSeed Co Ltd, Yantai 264006, Peoples R China 5.Jiangsu Baoyuan Biotechnol Co Ltd, Lianyungang 222144, Peoples R China 6.Jiangsu Haitai MariTech Co Ltd, Lianyungang 222144, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Xiaotong,Wang, Xia,Chen, Jieyu,et al. Functional analyses of TRAF6 gene in Argopecten scallops[J]. FISH & SHELLFISH IMMUNOLOGY,2024,147:9.
APA	Zhang, Xiaotong.,Wang, Xia.,Chen, Jieyu.,Chen, Min.,Lu, Xia.,...&Liu, Bo.(2024).Functional analyses of TRAF6 gene in Argopecten scallops.FISH & SHELLFISH IMMUNOLOGY,147,9.
MLA	Zhang, Xiaotong,et al."Functional analyses of TRAF6 gene in Argopecten scallops".FISH & SHELLFISH IMMUNOLOGY 147(2024):9.