New Near-Infrared Fluorescence Imaging Platform with Large Stokes Shift for Carboxylesterase 2 Detection in Thyroid Cancer and Inflammatory Diseases Diagnosis

doi:10.1021/acs.analchem.3c04399

	New Near-Infrared Fluorescence Imaging Platform with Large Stokes Shift for Carboxylesterase 2 Detection in Thyroid Cancer and Inflammatory Diseases Diagnosis
	Wang, Xiaochun 1,2; Gao, Jian 2; Fan, Chuanfeng 2; Gao, Yingkai 2; Yang, Xintong 2; Chen, Lingxin 3,4,5
刊名	ANALYTICAL CHEMISTRY
	2024-02-19
卷号	96 期号:9 页码:3772-3779
ISSN号	0003-2700
DOI	10.1021/acs.analchem.3c04399
通讯作者	Wang, Xiaochun(wangwxc@qust.edu.cn)
英文摘要	Development of new near-infrared fluorophores is one of the eternal themes in the field of biosensing and biological imaging. In this work, we constructed a novel fluorophore platform MOR by replacing methylindole of hemicyanine fluorophore (CyR) with benzoxazole to acquire better fluorescence characteristics. Based on the platform, a near infrared (NIR) fluorescent probe MOR-CES2 was synthesized for the specific "off-on" response to carboxylesterase 2 (CES2). The probe exhibited excellent properties including near-infrared emission (735 nm), large Stokes shift (105 nm), high sensitivity (LOD, 0.3 ng/mL), and rapid response (15 min). The successful application of MOR-CES2 in biological imaging of CES2 in mice with thyroid cancer and inflammatory bowel disease demonstrated that the probe could identify cancer cells and tissues and sensitively respond to inflammation. The results proved the potency of MOR-CES2 as an efficient imaging tool to assist in the surgical resection of CES2-related tumors.
WOS关键词	CATALYTIC-PROPERTIES ; LIVING CELLS ; PROBE ; ACTIVATION ; EXPRESSION ; ISOZYMES
WOS研究方向	Chemistry
语种	英语
WOS记录号	WOS:001166901900001
资助机构	National Natural Science Foundation of China ; Start-Up Fund of Qingdao University of Science and Technology
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/34879]
专题	中国科学院烟台海岸带研究所
通讯作者	Wang, Xiaochun
作者单位	1.Anshan Normal Univ, Sch Chem & Life Sci, Liaoning Key Lab Dev & Utilizat Nat Prod Act Mol, Anshan 114007, Peoples R China 2.Qingdao Univ Sci & Technol, Key Lab Opt Elect Sensing & Analyt Chem Life Sci, Shandong Prov Key Lab Biochem Engn, MOE,Coll Marine Sci & Biol Engn,Qingdao Nucl Acid, Qingdao 266042, Peoples R China 3.Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res,Res Ctr Coastal Envir, Yantai 264003, Peoples R China 4.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China 5.Shaoxing Univ, Coll Chem & Chem Engn, Shaoxing 312000, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xiaochun,Gao, Jian,Fan, Chuanfeng,et al. New Near-Infrared Fluorescence Imaging Platform with Large Stokes Shift for Carboxylesterase 2 Detection in Thyroid Cancer and Inflammatory Diseases Diagnosis[J]. ANALYTICAL CHEMISTRY,2024,96(9):3772-3779.
APA	Wang, Xiaochun,Gao, Jian,Fan, Chuanfeng,Gao, Yingkai,Yang, Xintong,&Chen, Lingxin.(2024).New Near-Infrared Fluorescence Imaging Platform with Large Stokes Shift for Carboxylesterase 2 Detection in Thyroid Cancer and Inflammatory Diseases Diagnosis.ANALYTICAL CHEMISTRY,96(9),3772-3779.
MLA	Wang, Xiaochun,et al."New Near-Infrared Fluorescence Imaging Platform with Large Stokes Shift for Carboxylesterase 2 Detection in Thyroid Cancer and Inflammatory Diseases Diagnosis".ANALYTICAL CHEMISTRY 96.9(2024):3772-3779.