Tetrahedral DNA framework assisted rotational paper-based analytical device for differential detection of SARS-CoV-2 and influenza A H1N1 virus

doi:10.1016/j.microc.2022.108304

	Tetrahedral DNA framework assisted rotational paper-based analytical device for differential detection of SARS-CoV-2 and influenza A H1N1 virus
	Li, Fengling 1; Qi, Ji 3; Ren, Zengzheng 1; Hu, Xiaoli 1; Chen, Yan 2; Li, Bowei 3; Fu, Xiuli 1
刊名	MICROCHEMICAL JOURNAL
	2023-02-01
卷号	185 页码:7
关键词	Tetrahedral DNA framework Paper-based analytical device SARS-CoV-2
ISSN号	0026-265X
DOI	10.1016/j.microc.2022.108304
通讯作者	Chen, Yan(ychen0612@163.com) ; Li, Bowei(bwli@yic.ac.cn) ; Fu, Xiuli(fuxiuli@ytu.edu.cn)
英文摘要	Coronavirus disease 2019 (COVID-19) and influenza A are two respiratory infectious diseases with similar clinical manifestations. Because of the complex global epidemic situation of COVID-19, the distinction and diagnosis of COVID-19 and influenza A infected persons is crucial for epidemic prevention and control. In this study, tetrahedral DNA framework (TDF) was combined with a rotational paper-based analytical device, and the color change generated by the reaction between horseradish peroxidase (HRP) and 3,3 ' 5,5 '-tetramethylbenzidine (TMB)-H2O2 was used for grayscale signal analysis by ImageJ software. The quantitative detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and influenza A H1N1 virus were realized simulta-neously. Under the optimal conditions, the paper-based analytical device showed a good linear relationship between the two viruses in the range of 10-14-10-8g/mL, and the two viruses were not affected by cross reaction. This sensor provides a convenient and reliable method for clinical rapid differentiation and diagnosis of COVID-19 and influenza A.
WOS关键词	NANOSTRUCTURE ; MICROFLUIDICS ; PLATFORM
WOS研究方向	Chemistry
语种	英语
WOS记录号	WOS:000905569200001
资助机构	Qingchuang Science and Technology Program of Shandong Province in China ; National Natural Science Foundation of China ; Shandong Provincial Natural Science Foundation Key Project
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/34811]
专题	中国科学院烟台海岸带研究所
通讯作者	Chen, Yan; Li, Bowei; Fu, Xiuli
作者单位	1.Yantai Univ, Sch Chem & Chem Engn, Yantai 264005, Peoples R China 2.Shandong Agr & Engn Univ, Sch Resources & Environm Engn, Jinan 250100, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Li, Fengling,Qi, Ji,Ren, Zengzheng,et al. Tetrahedral DNA framework assisted rotational paper-based analytical device for differential detection of SARS-CoV-2 and influenza A H1N1 virus[J]. MICROCHEMICAL JOURNAL,2023,185:7.
APA	Li, Fengling.,Qi, Ji.,Ren, Zengzheng.,Hu, Xiaoli.,Chen, Yan.,...&Fu, Xiuli.(2023).Tetrahedral DNA framework assisted rotational paper-based analytical device for differential detection of SARS-CoV-2 and influenza A H1N1 virus.MICROCHEMICAL JOURNAL,185,7.
MLA	Li, Fengling,et al."Tetrahedral DNA framework assisted rotational paper-based analytical device for differential detection of SARS-CoV-2 and influenza A H1N1 virus".MICROCHEMICAL JOURNAL 185(2023):7.