Systematic Survey of the Regulatory Networks of the Long Noncoding RNA BANCR in Cervical Cancer Cells br

doi:10.1021/acs.jproteome.2c00009

	Systematic Survey of the Regulatory Networks of the Long Noncoding RNA BANCR in Cervical Cancer Cells br
	Wang, Bing 1,2,3; Wang, Min 4; Jia, Shuzhao 4; Li, Tao 1,2; Yang, Mingkun 1,2,3; Ge, Feng 1,2,3
刊名	JOURNAL OF PROTEOME RESEARCH
	2022-04-01
卷号	21 期号:4 页码:1137-1152
关键词	long noncoding RNAs (lncRNAs) BRAF-activated noncoding RNA (BANCR) quantitative proteomics cAMP regulatory element binding protein (CREB1) miR-582-5p
ISSN号	1535-3893
DOI	10.1021/acs.jproteome.2c00009
通讯作者	Yang, Mingkun(yangmingkun@ihb.ac.cn) ; Ge, Feng(gefeng@ihb.ac.cn)
英文摘要	Long noncoding RNAs (lncRNAs) are increasingly recog-nized as functional regulators of human cancers. BRAF-activated noncodingRNA (BANCR), an oncogenic lncRNA, has a carcinogenic effect on manytypes of cancers. However, the clinical significance and molecularmechanisms of BANCR in cervical cancer are still unclear. Here, wegenerated BANCR knockout cell linesviaCRISPR/Cas9 editing andrevealed that BANCR plays roles in the apoptosis, proliferation, andmigration of HeLa cells. A quantitative proteomics strategy was employed toglobally identify BANCR-regulated proteins in HeLa cells. In total, weidentified 569 differentially expressed proteins (DEPs) upon knockout ofBANCR in HeLa cells. Bioinformatic analysis revealed that these DEPs wereinvolved in diverse cellular pathways. Functional studies revealed thatBANCR exerts its effects on the proliferation and apoptosis of HeLa cellsthrough the regulation of cAMP-responsive element binding protein 1(CREB1) expression. Mechanistically, BANCR could inhibit the expression of miR-582-5p, and CREB1 is a direct target of miR-582-5p; therefore, BANCR may exert its function by regulating CREB1 expressionviatargeting miR-582-5p in cervical cancer cells.Collectively, this study established a proteome-wide BANCR regulatory network, which provides novel insight into the molecularpathogenesis of cervical cancer and can serve as a basis for the development of targeted therapies.
WOS关键词	COMPETING ENDOGENOUS RNA ; PROLIFERATION ; GROWTH ; SUPPRESSES ; EXPRESSION ; INVASION ; HOTAIR ; CREB ; HEMATOPOIESIS ; TUMORIGENESIS
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
WOS记录号	WOS:000784194300026
资助机构	Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Chinese Academy of Sciences Key Technology Talent Program
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/34806]
专题	中国科学院烟台海岸带研究所
通讯作者	Yang, Mingkun; Ge, Feng
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Anal & Testing Ctr, Inst Hydrobiol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Wang, Bing,Wang, Min,Jia, Shuzhao,et al. Systematic Survey of the Regulatory Networks of the Long Noncoding RNA BANCR in Cervical Cancer Cells br[J]. JOURNAL OF PROTEOME RESEARCH,2022,21(4):1137-1152.
APA	Wang, Bing,Wang, Min,Jia, Shuzhao,Li, Tao,Yang, Mingkun,&Ge, Feng.(2022).Systematic Survey of the Regulatory Networks of the Long Noncoding RNA BANCR in Cervical Cancer Cells br.JOURNAL OF PROTEOME RESEARCH,21(4),1137-1152.
MLA	Wang, Bing,et al."Systematic Survey of the Regulatory Networks of the Long Noncoding RNA BANCR in Cervical Cancer Cells br".JOURNAL OF PROTEOME RESEARCH 21.4(2022):1137-1152.