Surface-Engineered Gold Nanoclusters for Stimulated Emission Depletion and Correlated Light and Electron Microscopy Imaging

doi:10.1021/acs.analchem.1c03935

	Surface-Engineered Gold Nanoclusters for Stimulated Emission Depletion and Correlated Light and Electron Microscopy Imaging
	Yang, Hongwei 1,2; Wu, Yayun 1; Ruan, Hefei 1; Guo, Feng 3; Liang, Yuxin 1,2; Qin, Gege 1,2; Liu, Xiaolong 1,2; Zhang, Zhen 1; Yuan, Jinghe 1; Fang, Xiaohong 1,4
刊名	ANALYTICAL CHEMISTRY
	2022-02-10
页码	9
ISSN号	0003-2700
DOI	10.1021/acs.analchem.1c03935
通讯作者	Yuan, Jinghe(jhyuan@iccas.ac.cn) ; Fang, Xiaohong(xfang@ccas.ac.cn)
英文摘要	Stimulated emission depletion (STED) nanoscopy is an emerging super-resolution imaging platform for the study of the cellular structure. Developing suitable fluorescent probes of small size, good photostability, and easy functionalization is still in demand. Herein, we introduce a new type of surface-engineered gold nanoclusters (Au NCs) that are ultrasmall (1.7 nm) and ultrabright (QY = 60%) for STED bioimaging. A rigid shell formed by L-arginine (L-Arg) and 6-aza-2thiothymine (ATT) on the Au NC surface enables not only its strong fluorescence in aqueous solution but also its easy chemical modification for specific biomolecule labeling. Au NCs show remarkable performance as STED nanoprobes, including high depletion efficiency, good photobleaching resistance, and low saturation intensity. Super-resolution imaging has been achieved with these Au NCs, and targeted nanoscopic imaging of cellular tubulin has been demonstrated. Moreover, the circular structure of lysosomes in live cells has been revealed. As a Au NC is also an ideal probe for electron microscopy, dual imaging of A beta 42 aggregates with the single labeling probe of Au NCs has been realized in correlative light and electron microscopy (CLEM). This work reports, for the first time, the application of Au NCs as a novel probe in STED and CLEM imaging. With their excellent properties, Au NCs show promising potential for nanoscale bioimaging.
WOS关键词	SUPERRESOLUTION ; NANOPARTICLES ; FLUORESCENCE ; PROBES
WOS研究方向	Chemistry
语种	英语
WOS记录号	WOS:000757849700001
资助机构	National Natural Science Foundation of China ; Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/34784]
专题	中国科学院烟台海岸带研究所
通讯作者	Yuan, Jinghe; Fang, Xiaohong
作者单位	1.Chinese Acad Sci, CAS Res Educ Ctr Excellence Mol Sci, Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Anal & Testing Ctr, Inst Proc Engn, Beijing 100190, Peoples R China 4.Chinese Acad Sci, Inst Canc & Basic Med, Hangzhou 310022, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Yang, Hongwei,Wu, Yayun,Ruan, Hefei,et al. Surface-Engineered Gold Nanoclusters for Stimulated Emission Depletion and Correlated Light and Electron Microscopy Imaging[J]. ANALYTICAL CHEMISTRY,2022:9.
APA	Yang, Hongwei.,Wu, Yayun.,Ruan, Hefei.,Guo, Feng.,Liang, Yuxin.,...&Fang, Xiaohong.(2022).Surface-Engineered Gold Nanoclusters for Stimulated Emission Depletion and Correlated Light and Electron Microscopy Imaging.ANALYTICAL CHEMISTRY,9.
MLA	Yang, Hongwei,et al."Surface-Engineered Gold Nanoclusters for Stimulated Emission Depletion and Correlated Light and Electron Microscopy Imaging".ANALYTICAL CHEMISTRY (2022):9.