莱茵衣藻蓝光受体植物类型隐花色素CRY突变体的表型鉴定

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	莱茵衣藻蓝光受体植物类型隐花色素CRY突变体的表型鉴定
	李旺宁 2; 张豪杰 2; 李亚男 2; 梁梦静 2; 季春丽 2; 张春辉 2; 李润植 2; 崔玉琳 1; 秦松 1; 崔红利 2
刊名	生物技术通报
	2023
卷号	39 期号:2 页码:243-253
关键词	莱茵衣藻隐花色素蓝光突变株表型鉴定
ISSN号	1002-5464
其他题名	Phenotypic Characterization of Blue Photoreceptor Plant Type Cryptochrome CRY Mutant in Chlamydomonas reinhardtii
文献子类	期刊论文
英文摘要	The phenotypic differences of Chlamydomonas reinhardtii wild strain(CC5325)and CRY mutant strain(crcry)under normal CK(Control)and Blue BL(Blue light)cultures were investigated. The PCR verification showed that the crcry mutant was inserted with paromomycin resistance gene AphVIII expression box in the coding region. AphVIII resistance gene was inserted into crcry mutant and successfully expressed in plate and liquid culture system with paromomycin as screening condition. Phenotypic identification showed that there were no significant differences in growth, pigment, photosynthesis and lipid synthesis between wild strain CC5325 and mutant strain crcry under CK culture condition. However, under BL condition, the growth of mutant crcry was significantly inhibited, and the color of algae liquid turned yellow. Chlorophyll a, chlorophyll b and total pigment contents of unit cells decreased significantly, while total carotenoid contents increased significantly. The photosynthetic system was severely suppressed and the total lipid content significantly reduced. In conclusion, plant cry is involved in the blue light response of C. reinhardtii.
语种	中文
CSCD记录号	CSCD:7420723
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/34217]
专题	海岸带生物学与生物资源利用重点实验室
作者单位	1.中国科学院烟台海岸带研究所海岸带生物学与生物资源保护重点实验室,烟台264003 2.山西农业大学农学院分子农业与生物能源研究所,太谷030801;
推荐引用方式 GB/T 7714	李旺宁,张豪杰,李亚男,等. 莱茵衣藻蓝光受体植物类型隐花色素CRY突变体的表型鉴定[J]. 生物技术通报,2023,39(2):243-253.
APA	李旺宁.,张豪杰.,李亚男.,梁梦静.,季春丽.,...&崔红利.(2023).莱茵衣藻蓝光受体植物类型隐花色素CRY突变体的表型鉴定.生物技术通报,39(2),243-253.
MLA	李旺宁,et al."莱茵衣藻蓝光受体植物类型隐花色素CRY突变体的表型鉴定".生物技术通报 39.2(2023):243-253.