基于环境DNA宏条形码技术研究黄河三角洲典型潮沟系统鱼类多样性及其对水文连通性的响应

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	基于环境DNA宏条形码技术研究黄河三角洲典型潮沟系统鱼类多样性及其对水文连通性的响应
	董志远 2,3; 陈琳琳 2,3; 张乃鹏 4; 陈莉 1,2; 孙德斌 2,3; 倪艳梅 1,2; 李宝泉 2,3
刊名	生物多样性
	2023
卷号	31 期号:7 页码:96-110
关键词	环境DNA宏条形码鱼类多样性水文连通黄河三角洲潮沟系统
ISSN号	1005-0094
其他题名	Response of fish diversity to hydrological connectivity of typical tidal creek system in the Yellow River Delta based on environmental DNA metabarcoding
文献子类	期刊论文
英文摘要	Aims:Hydrological connectivity is essential for maintaining biodiversity in coastal wetlands,and it is important to understand the response of fish diversity,as a major component of biodiversity,to different hydrological connectivity intensities.This study aimed to investigate the response of fish diversity to hydrological connectivity in the typical tidal creek system and verify the applicability of eDNA metabarcoding for monitoring and conserving fish diversity in the Yellow River Delta.Methods:Water samples were collected from six sites in a typical tidal creek system in the Yellow River Delta in September 2022.Fish diversity was analyzed by using eDNA metabarcoding,in which,eDNA extracted from the water samples source were amplified by PCR using the 12S rRNA classical fish primers MiFish-E and high-throughput sequencing was performed by Illumina Miseq.The OTU representative sequences were aligned to the NCBI nucleotide sequence database to obtain species taxonomic annotation information.Thirteen environmental physicochemical indicators were measured in the water column.The relationship between fish communities and environmental factors was explored based on redundancy analysis.Results:A total of 55 fish species were detected,including 27 native and 28 non-native fish species,and Perciformes dominated the fish composition.The fishes with high sequence abundance at each sample site included Acanthogobius hasta,Planiliza haematocheilu,Acanthogobius elongatus,etc.Fish diversity of the tidal creeks with different hydrological connectivity was different.Significantly higher community diversity and richness index,the highest number of species and a more even distribution of the number of individual species were found in the secondary tidal creek than in the other two tidal creeks.SiO_3~(2-)-Si,NO_3~--N,pH,salinity,NH_4~+-N,and dissolved oxygen were significantly correlated (P <0.05) with fish community structure shown by RDA analysis.Acanthogobius hasta with higher sequence abundance was positively correlated with silicate,and the sequence abundance of Planiliza haematocheilus was negatively correlated with salinity.Conclusion:This study confirmed the feasibility of using environmental DNA metabarcoding to monitor fish diversity of typical tidal water bodies in the Yellow River estuary by comparing it with previous traditional sampling data and indicated that hydrological connectivity of tidal creek systems has a significant impact on fish community structure and diversity.The results of this study are helpful to further understand the influence mechanism of coastal wetland hydrological connectivity on biological communities.
语种	中文
CSCD记录号	CSCD:7522125
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/34212]
专题	海岸带生物学与生物资源利用重点实验室
作者单位	1.烟台大学海洋学院,山东烟台264003; 2.中国科学院烟台海岸带研究所海岸带生物学与生物资源保护实验室,山东烟台264003; 3.中国科学院大学,北京100049; 4.东营市水文中心,山东东营257000
推荐引用方式 GB/T 7714	董志远,陈琳琳,张乃鹏,等. 基于环境DNA宏条形码技术研究黄河三角洲典型潮沟系统鱼类多样性及其对水文连通性的响应[J]. 生物多样性,2023,31(7):96-110.
APA	董志远.,陈琳琳.,张乃鹏.,陈莉.,孙德斌.,...&李宝泉.(2023).基于环境DNA宏条形码技术研究黄河三角洲典型潮沟系统鱼类多样性及其对水文连通性的响应.生物多样性,31(7),96-110.
MLA	董志远,et al."基于环境DNA宏条形码技术研究黄河三角洲典型潮沟系统鱼类多样性及其对水文连通性的响应".生物多样性 31.7(2023):96-110.