Highly efficient removal of patulin using immobilized enzymes of Pseudomonas aeruginosa TF-06 entrapped in calcium alginate beads

doi:10.1016/j.foodchem.2021.131973

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院北方资源植物重点实验室

	Highly efficient removal of patulin using immobilized enzymes of Pseudomonas aeruginosa TF-06 entrapped in calcium alginate beads
	Xing, Mengyang 2; Chen, Yong ; Li, Boqiang ; Tian, Shiping 2
刊名	FOOD CHEMISTRY
	2022
卷号	377
关键词	Patulin degradation Pseudomonas aeruginosa Immobilized enzyme Calcium alginate beads Apple juice
ISSN号	0308-8146
DOI	10.1016/j.foodchem.2021.131973
文献子类	Article
英文摘要	Patulin is a toxic secondary metabolite produced by several moulds, which contaminates fruits and their products posing serious threats to human health. Though several microorganisms and enzymes have been reported to effectively degrade patulin, separation of them from fruit juice challenges the commercial applications. Here, a Pseudomonas aeruginosa strain TF-06 was isolated, its patulin degradation mechanism and optimum conditions for enzyme immobilization were investigated. The results indicated that TF-06 could degrade patulin into noncytotoxic E/Z-ascladiol mainly by the activity of intracellular enzymes. For easy separation of enzymes, calcium alginate was selected for immobilization of intracellular enzymes from TF-06. The immobilized enzyme beads were effective in detoxification of patulin in apple juice. The mitigation rate was reached 95%, while there was no negative effect on juice quality. The study provides a promising way to resolve the issue of enzyme separation during mycotoxin biological detoxification in fruit juice.
学科主题	Chemistry, Applied ; Food Science & Technology ; Nutrition & Dietetics
电子版国际标准刊号	1873-7072
出版地	OXFORD
WOS关键词	MYCOTOXIN PATULIN ; BIODEGRADATION ; INSIGHTS ; CELLS ; JUICE ; SOIL
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000740932400006
资助机构	Strategic Priority Research Program of the Chinese Academy of Sciences [XDA26040202] ; National Natural Science Foundation of China [31930086] ; National Key R&D Program of China [2016YFD0400902] ; Youth Innovation Promotion Association, CAS [Y201919]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/29024]
专题	中科院北方资源植物重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Innovat Acad Seed Design, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Xing, Mengyang,Chen, Yong,Li, Boqiang,et al. Highly efficient removal of patulin using immobilized enzymes of Pseudomonas aeruginosa TF-06 entrapped in calcium alginate beads[J]. FOOD CHEMISTRY,2022,377.
APA	Xing, Mengyang,Chen, Yong,Li, Boqiang,&Tian, Shiping.(2022).Highly efficient removal of patulin using immobilized enzymes of Pseudomonas aeruginosa TF-06 entrapped in calcium alginate beads.FOOD CHEMISTRY,377.
MLA	Xing, Mengyang,et al."Highly efficient removal of patulin using immobilized enzymes of Pseudomonas aeruginosa TF-06 entrapped in calcium alginate beads".FOOD CHEMISTRY 377(2022).