Cryo-electron microscopy structure of the intact photosynthetic light-harvesting antenna-reaction center complex from a green sulfur bacterium

doi:10.1111/jipb.13367

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	Cryo-electron microscopy structure of the intact photosynthetic light-harvesting antenna-reaction center complex from a green sulfur bacterium
	Chen, Jing-Hua ; Wang, Weiwei ; Wang, Chen 2,3; Kuang, Tingyun ; Shen, Jian-Ren ; Zhang, Xing 2,3,5
刊名	JOURNAL OF INTEGRATIVE PLANT BIOLOGY
	2023
卷号	65 期号:1 页码:223-234
关键词	cryo-electron microscopy energy transfer FMO protein green sulfur bacterium photosynthesis reaction center
ISSN号	1672-9072
DOI	10.1111/jipb.13367
文献子类	Article
英文摘要	The photosynthetic reaction center complex (RCC) of green sulfur bacteria (GSB) consists of the membrane-imbedded RC core and the peripheric energy transmitting proteins called Fenna-Matthews-Olson (FMO). Functionally, FMO transfers the absorbed energy from a huge peripheral light-harvesting antenna named chlorosome to the RC core where charge separation occurs. In vivo, one RC was found to bind two FMOs, however, the intact structure of RCC as well as the energy transfer mechanism within RCC remain to be clarified. Here we report a structure of intact RCC which contains a RC core and two FMO trimers from a thermophilic green sulfur bacterium Chlorobaculum tepidum at 2.9 angstrom resolution by cryo-electron microscopy. The second FMO trimer is attached at the cytoplasmic side asymmetrically relative to the first FMO trimer reported previously. We also observed two new subunits (PscE and PscF) and the N-terminal transmembrane domain of a cytochrome-containing subunit (PscC) in the structure. These two novel subunits possibly function to facilitate the binding of FMOs to RC core and to stabilize the whole complex. A new bacteriochlorophyll (numbered as 816) was identified at the interspace between PscF and PscA-1, causing an asymmetrical energy transfer from the two FMO trimers to RC core. Based on the structure, we propose an energy transfer network within this photosynthetic apparatus.
学科主题	Biochemistry & Molecular Biology ; Plant Sciences
电子版国际标准刊号	1744-7909
出版地	HOBOKEN
WOS关键词	CENTER CORE COMPLEX ; 3-DIMENSIONAL STRUCTURE ; CHLOROBACULUM-TEPIDUM ; CHLOROBIUM-TEPIDUM ; ELECTRON-TRANSFER ; PHOTOSYSTEM-I ; FMO-PROTEIN ; ENERGY ; ULTRASTRUCTURE ; CAROTENOIDS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	WILEY
WOS记录号	WOS:000905863500001
资助机构	National Natural Science Foundation of China [32100202] ; Natural Science Foundation of Zhejiang Province, China [LR22C010001] ; National Key Research and Development Program of China [2018YFA0507700, 2017YFA0504803] ; Fundamental Research Funds for the Central Universities [2018XZZX001-13]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/29090]
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Photosynth Res Ctr, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Zhejiang Univ, Sir Run Run Shaw Hosp, Dept Pathol, Sch Med, Hangzhou 310058, Peoples R China 3.Zhejiang Univ, Coll Life Sci, Hangzhou 310058, Peoples R China 4.Zhejiang Univ, Ctr Cryo Electron Microscopy, Sch Med, Hangzhou 310058, Peoples R China 5.Okayama Univ, Grad Sch Nat Sci & Technol, Okayama 7008530, Japan 6.Okayama Univ, Res Inst Interdisciplinary Sci, Okayama 7008530, Japan 7.Zhejiang Univ, Dept Biophys, Sch Med, Hangzhou 310058, Peoples R China
推荐引用方式 GB/T 7714	Chen, Jing-Hua,Wang, Weiwei,Wang, Chen,et al. Cryo-electron microscopy structure of the intact photosynthetic light-harvesting antenna-reaction center complex from a green sulfur bacterium[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2023,65(1):223-234.
APA	Chen, Jing-Hua,Wang, Weiwei,Wang, Chen,Kuang, Tingyun,Shen, Jian-Ren,&Zhang, Xing.(2023).Cryo-electron microscopy structure of the intact photosynthetic light-harvesting antenna-reaction center complex from a green sulfur bacterium.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,65(1),223-234.
MLA	Chen, Jing-Hua,et al."Cryo-electron microscopy structure of the intact photosynthetic light-harvesting antenna-reaction center complex from a green sulfur bacterium".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 65.1(2023):223-234.