The CDC48 complex mediates ubiquitin-dependent degradation of intra-chloroplast proteins in plants | |
Li, Jialong; Yuan, Jiarui1; Li, Yuhong1; Sun, Huilun1; Ma, Tingting; Huai, Junling; Yang, Wenqiang1; Zhang, Wenhao1,3; Lin, Rongcheng1 | |
刊名 | CELL REPORTS |
2022 | |
卷号 | 39期号:2 |
ISSN号 | 2211-1247 |
DOI | 10.1016/j.celrep.2022.110664 |
文献子类 | Article |
英文摘要 | Chloroplasts are the site of numerous biochemical reactions including photosynthesis, but they also produce reactive oxygen species (ROS) that negatively affect chloroplast integrity. The chaperone-like CDC48 com-plex plays critical roles in ubiquitin-dependent protein degradation in yeast and mammals, but its function in plants is largely unknown. Here, we show that defects in CDC48A and its cofactors UFD1 and NPL4 lead to the accumulation of ubiquitinated chloroplast proteins in Arabidopsis thaliana. We reveal that two plastid genome-encoded proteins, RbcL and AtpB, associate with the CDC48 complex. Strikingly, RbcL and AtpB are ubiquitinated and degraded by the 26S proteasome pathway upon ROS stress, and these processes are impaired by defects of the CDC48 complex. Functional analysis demonstrates that the CDC48 complex is required for plant tolerance to ROS. This study reveals a role for the plant CDC48 complex in modulating ubiquitin-dependent degradation of intra-chloroplast proteins in response to oxidative stress. |
学科主题 | Cell Biology |
出版地 | CAMBRIDGE |
WOS关键词 | E3 LIGASE ATCHIP ; QUALITY-CONTROL ; REACTIVE OXYGEN ; LYSINE UBIQUITINATION ; PROTEASOME SYSTEM ; FTSH PROTEASE ; RETROTRANSLOCATION ; BIOGENESIS ; SUBSTRATE ; REVEALS |
WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
语种 | 英语 |
出版者 | CELL PRESS |
WOS记录号 | WOS:000793180100001 |
资助机构 | National Key Research and Development Program of China [2020YFA0907600, 2019YFA0904600] ; National Natural Science Foundation of China [32030009] ; Strategic Priority Research Program of the Chinese Academy of Sciences [XDB27030205] |
内容类型 | 期刊论文 |
源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28758] |
专题 | 中科院光生物学重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Li, Jialong,Yuan, Jiarui,Li, Yuhong,et al. The CDC48 complex mediates ubiquitin-dependent degradation of intra-chloroplast proteins in plants[J]. CELL REPORTS,2022,39(2). |
APA | Li, Jialong.,Yuan, Jiarui.,Li, Yuhong.,Sun, Huilun.,Ma, Tingting.,...&Lin, Rongcheng.(2022).The CDC48 complex mediates ubiquitin-dependent degradation of intra-chloroplast proteins in plants.CELL REPORTS,39(2). |
MLA | Li, Jialong,et al."The CDC48 complex mediates ubiquitin-dependent degradation of intra-chloroplast proteins in plants".CELL REPORTS 39.2(2022). |
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