Exploring key developmental phases and phase-specific genes across the entirety of anther development in maize

doi:10.1111/jipb.13276

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	Exploring key developmental phases and phase-specific genes across the entirety of anther development in maize
	Han, Yingjia ; Hu, Mingjian 3,4; Ma, Xuxu ; Yan, Ge 2; Wang, Chunyu 2; Jiang, Siqi 2; Lai, Jinsheng 3,4; Zhang, Mei
刊名	JOURNAL OF INTEGRATIVE PLANT BIOLOGY
	2022
卷号	64 期号:7 页码:1394-1410
关键词	anther development maize male-sterile phase-specific genes transcriptome ZmDRP1
ISSN号	1672-9072
DOI	10.1111/jipb.13276
文献子类	Article
英文摘要	Anther development from stamen primordium to pollen dispersal is complex and essential to sexual reproduction. How this highly dynamic and complex developmental process is controlled genetically is not well understood, especially for genes involved in specific key developmental phases. Here we generated RNA sequencing libraries spanning 10 key stages across the entirety of anther development in maize (Zea mays). Global transcriptome analyses revealed distinct phases of cell division and expansion, meiosis, pollen maturation, and mature pollen, for which we detected 50, 245, 42, and 414 phase-specific marker genes, respectively. Phase-specific transcription factor genes were significantly enriched in the phase of meiosis. The phase-specific expression of these marker genes was highly conserved among the maize lines Chang7-2 and W23, indicating they might have important roles in anther development. We explored a desiccation-related protein gene, ZmDRP1, which was exclusively expressed in the tapetum from the tetrad to the uninucleate microspore stage, by generating knockout mutants. Notably, mutants in ZmDRP1 were completely male-sterile, with abnormal Ubisch bodies and defective pollen exine. Our work provides a glimpse into the gene expression dynamics and a valuable resource for exploring the roles of key phase-specific genes that regulate anther development.
学科主题	Biochemistry & Molecular Biology ; Plant Sciences
电子版国际标准刊号	1744-7909
出版地	HOBOKEN
WOS关键词	NICOTIANAMINE SYNTHASE GENES ; POLLEN WALL DEVELOPMENT ; TRANSCRIPTION FACTOR ; MALE-FERTILITY ; ZEA-MAYS ; FUNCTIONAL-CHARACTERIZATION ; MONOSACCHARIDE TRANSPORTER ; REPRODUCTIVE DEVELOPMENT ; SUCROSE TRANSPORTER ; MOLECULAR-CLONING
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	WILEY
WOS记录号	WOS:000822742000011
资助机构	Strategic Priority Research Program of the Chinese Academy of Sciences [XDA24010106] ; National Natural Science Foundation of China [32072075] ; National Center for Biotechnology Information's Short Read Archive under BioProject [PRJNA750514]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/28500]
专题	中科院植物分子生理学重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.China Agr Univ, Natl Maize Improvement Ctr China, Dept Plant Genet & Breeding, Beijing 100193, Peoples R China 3.China Agr Univ, Dept Plant Genet & Breeding, State Key Lab Plant Physiol & Biochem, Beijing 100193, Peoples R China 4.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Han, Yingjia,Hu, Mingjian,Ma, Xuxu,et al. Exploring key developmental phases and phase-specific genes across the entirety of anther development in maize[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2022,64(7):1394-1410.
APA	Han, Yingjia.,Hu, Mingjian.,Ma, Xuxu.,Yan, Ge.,Wang, Chunyu.,...&Zhang, Mei.(2022).Exploring key developmental phases and phase-specific genes across the entirety of anther development in maize.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,64(7),1394-1410.
MLA	Han, Yingjia,et al."Exploring key developmental phases and phase-specific genes across the entirety of anther development in maize".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 64.7(2022):1394-1410.