The methyl-CpG-binding domain family member PEM1 is essential for Ubisch body formation and pollen exine development in rice

doi:10.1111/tpj.15887

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院植物分子生理学重点实验室

	The methyl-CpG-binding domain family member PEM1 is essential for Ubisch body formation and pollen exine development in rice
	Song, Yunyun 1; Tang, Yongyan ; Liu, Lingtong ; Xu, Yunyuan 1,3; Wang, Tai 1,3
刊名	PLANT JOURNAL
	2022
卷号	111 期号:5 页码:1283-1295
关键词	MBD protein Ubisch body pollen exine pollen Oryza sativa
ISSN号	0960-7412
DOI	10.1111/tpj.15887
文献子类	Article
英文摘要	Pollen exine is composed of finely-organized nexine, bacula and tectum, and is crucial for pollen viability and function. Pollen exine development involves a complicated molecular network that coordinates the interaction between pollen and tapetal cells, as well as the biosynthesis, transport and assembly of sporopollenin precursors; however, our understanding of this network is very limited. Here, we report the roles of PEM1, a member of methyl-CpG-binding domain family, in rice pollen development. PEM1 expressed constitutively and, in anthers, its expression was detectable in tapetal cells and pollen. This predicted PEM1 protein of 240 kDa had multiple epigenetic-related domains. pem1 mutants exhibited abnormal Ubisch bodies, delayed exine occurrence and, finally, defective exine, including invisible bacula, amorphous and thickened nexine and tectum layer structures, and also had the phenotype of increased anther cuticle. The mutation in PEM1 did not affect the timely degradation of tapetum. Lipidomics revealed much higher wax and cutin contents in mutant anthers than in wild-type. Accordingly, this mutation up-regulated the expression of a set of genes implicated in transcriptional repression, signaling and diverse metabolic pathways. These results indicate that PEM1 mediates Ubisch body formation and pollen exine development mainly by negatively modulating the expression of genes. Thus, the PEM1-mediated molecular network represents a route for insights into mechanisms underlying pollen development. PEM1 may be a master regulator of pollen exine development.
学科主题	Plant Sciences
电子版国际标准刊号	1365-313X
出版地	HOBOKEN
WOS关键词	TRANSCRIPTION FACTOR ; TAPETUM DEVELOPMENT ; DNA METHYLATION ; GENETIC PATHWAY ; FLOWERING TIME ; MALE-STERILITY ; WALL FORMATION ; MALE-FERTILITY ; EXPRESSION ; PROTEIN
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	WILEY
WOS记录号	WOS:000823286900001
资助机构	Chinese Academy of Sciences [XDA24010103] ; National Natural Science Foundation of China [32000247]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/28489]
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Song, Yunyun,Tang, Yongyan,Liu, Lingtong,et al. The methyl-CpG-binding domain family member PEM1 is essential for Ubisch body formation and pollen exine development in rice[J]. PLANT JOURNAL,2022,111(5):1283-1295.
APA	Song, Yunyun,Tang, Yongyan,Liu, Lingtong,Xu, Yunyuan,&Wang, Tai.(2022).The methyl-CpG-binding domain family member PEM1 is essential for Ubisch body formation and pollen exine development in rice.PLANT JOURNAL,111(5),1283-1295.
MLA	Song, Yunyun,et al."The methyl-CpG-binding domain family member PEM1 is essential for Ubisch body formation and pollen exine development in rice".PLANT JOURNAL 111.5(2022):1283-1295.