Localization of stomatal lineage proteins reveals contrasting planar polarity patterns in Arabidopsis cotyledons

doi:10.1016/j.cub.2022.09.049

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	Localization of stomatal lineage proteins reveals contrasting planar polarity patterns in Arabidopsis cotyledons
	Fozard, John A.; Yu, Man ; Bezodis, William ; Cheng, Jie 1,3; Spooner, Jamie ; Mansfield, Catherine ; Chan, Jordi ; Coen, Enrico
刊名	CURRENT BIOLOGY
	2022
卷号	32 期号:22 页码:4967-4974
ISSN号	0960-9822
DOI	10.1016/j.cub.2022.09.049
文献子类	Article
英文摘要	Many plant cells exhibit polarity, revealed by asymmetric localization of specific proteins within each cell.1-6 Polarity is typically coordinated between cells across a tissue, raising the question of how coordination is achieved. One hypothesis is that mechanical stresses provide cues.7 This idea gains support from experiments in which cotyledons were mechanically stretched transversely to their midline.8 These previously published re-sults showed that without applied tension, the stomatal lineage cell polarity marker, BREVIS RADIX-LIKE 2 (BRXL2), exhibited no significant excess in the transverse orientation. By contrast, 7 h after stretching, BRXL2 polarity distribution exhibited transverse excess, aligned with the stretch direction. These stretching ex-periments involved statistical comparisons between snapshots of stretched and unstretched cotyledons, with different specimens being imaged in each case.8 Here, we image the same cotyledon before and after stretching and find no evidence for reorientation of polarity. Instead, statistical analysis shows that cotyledons contain a pre-existing transverse excess in BRXL2 polarity orientation that is not significantly modified by applied tension. The transverse excess reflects BRLX2 being preferentially localized toward the medial side of the cell, nearer to the cotyledon midline, creating a weak medial bias. A second polarity marker, BREAKING OF ASYMMETRY IN THE STOMATAL LINEAGE (BASL), also exhibits weak medial bias in stomatal lineages, whereas ectopic expres-sion of BASL in non-stomatal cells exhibits strong proximal bias, as previously observed in rosette leaves. This proximal bias is also unperturbed by applied tension. Our findings therefore show that cotyledons contain two near-orthogonal coordinated biases in planar polarity: mediolateral and proximodistal.
学科主题	Biochemistry & Molecular Biology ; Biology ; Cell Biology
电子版国际标准刊号	1879-0445
出版地	CAMBRIDGE
WOS关键词	ASYMMETRIC CELL-DIVISION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	CELL PRESS
WOS记录号	WOS:000901528800011
资助机构	UK Biotechnology and Biological Sciences Research Council ; [BB/W007924/1] ; [BBS/E/J/000PR9787] ; [BBS/E/J/00000152] ; [BB/L008920/1] ; [BBS/E/J/000PR9789]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/28824]
专题	系统与进化植物学国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, CAS Ctr Excellence Mol Plant Sci, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China 3.John Innes Ctr, Dept Cell & Dev Biol, Norwich NR4 7UH, England
推荐引用方式 GB/T 7714	Fozard, John A.,Yu, Man,Bezodis, William,et al. Localization of stomatal lineage proteins reveals contrasting planar polarity patterns in Arabidopsis cotyledons[J]. CURRENT BIOLOGY,2022,32(22):4967-4974.
APA	Fozard, John A..,Yu, Man.,Bezodis, William.,Cheng, Jie.,Spooner, Jamie.,...&Coen, Enrico.(2022).Localization of stomatal lineage proteins reveals contrasting planar polarity patterns in Arabidopsis cotyledons.CURRENT BIOLOGY,32(22),4967-4974.
MLA	Fozard, John A.,et al."Localization of stomatal lineage proteins reveals contrasting planar polarity patterns in Arabidopsis cotyledons".CURRENT BIOLOGY 32.22(2022):4967-4974.