Characteristics of two crustins from Alvinocaris longirostris in Hydrothermal Vents

doi:10.3390/md19110600

CORC > 深海科学与工程研究所 > 中国科学院深海科学与工程研究所 > 深海科学研究部 > 深海生物学研究室 > 深海生物蛋白质组学及分子生物学研究组

	Characteristics of two crustins from Alvinocaris longirostris in Hydrothermal Vents
	Guo LL(郭璐璐); Wang SL(王少露); Zhu FC(朱方超); Xue F(薛峰); He LS(贺丽生)
刊名	Marine drugs
	2021
卷号	19 期号:11 页码:13
关键词	crustins antibacterial peptides hydrothermal vent anti-Gram-negative bacteria Al-crus 3 and Al-crus 7
DOI	10.3390/md19110600
通讯作者	He LS(贺丽生)
英文摘要	Crustins are widely distributed among different crustacean groups. They are characterized by a whey acidic protein (WAP) domain, and most examined Crustins show activity against Gram-positive bacteria. This study reports two Crustins, Al-crus 3 and Al-crus 7, from hydrothermal vent shrimp, Alvinocaris longirostris. Al-crus 3 and Al-crus 7 belong to Crustin Type IIa, with a similarity of about 51% at amino acid level. Antibacterial assays showed that Al-crus 3 mainly displayed activity against Gram-positive bacteria with MIC50 values of 10-25 mu M. However, Al-crus 7 not only displayed activity against Gram-positive bacteria but also against Gram-negative bacteria Imipenem-resistant Acinetobacter baumannii, in a sensitive manner. Notably, in the effective antibacterial spectrum, Methicillin-sensitive Staphylococcus aureus, Escherichia coli (ESBLs) and Imipenem-resistant A. baumannii were drug-resistant pathogens. Narrowing down the sequence to the WAP domain, Al-crusWAP 3 and Al-crusWAP 7 demonstrated antibacterial activities but were weak. Additionally, the effects on bacteria did not significantly change after they were maintained at room temperature for 48 h. This indicated that Al-crus 3 and Al-crus 7 were relatively stable and convenient for transportation. Altogether, this study reported two new Crustins with specific characteristics. In particular, Al-crus 7 inhibited Gram-negative imipenem-resistant A. baumannii.
资助项目	Institute and Local Government Cooperation Projects of Sanya[2019YD03] ; Major Scientific and Technological Projects of Hainan Province[ZDKJ2019011] ; National Key Research and Development Program of China[2018YFC0309804]
WOS关键词	BLACK TIGER SHRIMP ; RECOMBINANT EXPRESSION ; ANTIMICROBIAL PEPTIDES ; MOLECULAR-CLONING ; PENAEUS-MONODON ; ANTIBACTERIAL PEPTIDE ; SHORE CRAB ; HEMOCYTES ; INFECTION ; PROTEIN
WOS研究方向	Pharmacology & Pharmacy
语种	英语
出版者	MDPI
WOS记录号	WOS:000806913700001
资助机构	Institute and Local Government Cooperation Projects of Sanya ; Major Scientific and Technological Projects of Hainan Province ; National Key Research and Development Program of China
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.idsse.ac.cn/handle/183446/9046]
专题	深海科学研究部_深海生物学研究室_深海生物蛋白质组学及分子生物学研究组
通讯作者	He LS(贺丽生)
作者单位	1.北京大学 2.中国科学院深海科学与工程研究所
推荐引用方式 GB/T 7714	Guo LL,Wang SL,Zhu FC,et al. Characteristics of two crustins from Alvinocaris longirostris in Hydrothermal Vents[J]. Marine drugs,2021,19(11):13.
APA	Guo LL,Wang SL,Zhu FC,Xue F,&He LS.(2021).Characteristics of two crustins from Alvinocaris longirostris in Hydrothermal Vents.Marine drugs,19(11),13.
MLA	Guo LL,et al."Characteristics of two crustins from Alvinocaris longirostris in Hydrothermal Vents".Marine drugs 19.11(2021):13.