A Novel CYP4 Gene Identified in the Polychaete Sternaspis scutata and Its Transcriptional Levels along the Coasts of the Liaodong Peninsula

doi:10.3390/w14213489

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室

	A Novel CYP4 Gene Identified in the Polychaete Sternaspis scutata and Its Transcriptional Levels along the Coasts of the Liaodong Peninsula
	Yu, Zhenglin 2; Qi, Aimin 1; Wang, Lili 1; Shan, Encui 2; Li, Dongmei 1; Yang, Xiaolong 1; Zhang, Anguo 1
刊名	WATER
	2022-11-01
卷号	14 期号:21 页码:12
关键词	cytochrome P450 polychaetes Sternaspis sculata transcriptional response PAHs field study
DOI	10.3390/w14213489
通讯作者	Wang, Lili(llwang@nmemc.org.cn)
英文摘要	Polychaetes (such as Sternaspis sculata) can biotransform polycyclic aromatic hydrocarbons (PAHs) in the environment, and the cytochrome P450 (CYP) enzyme is the key substance in this process. Here, a novel CYP gene was identified from S. sculata, a marine polychaete that is abundantly distributed along marine coasts worldwide. The full-length cDNA of the new CYP is a 1829 bp, encoding a protein of 509 amino acids. The deduced amino acid sequence of the CYP gene contains the conserved motifs of the P450 family (FxxGxxxCxG) and the characteristic sequences of the CYP4 family (EVDTFMFEGHDTT), showing high similarity to CYP4V from marine polychaetes. Therefore, the cDNA sequence might belong to the subfamily of CYP4V, tentatively named SsCYP4V. The expression levels of SsCYP4V in the field were detected in S. sculata sampled from six sites along the coasts of the Liaodong Peninsula. The results showed that the relative expression levels of SsCYP4V in S. sculata were significantly different among the sampling sites; however, no positive relationship was found between SsCYP4V expression levels and PAHs concentrations in the sediments. These findings suggested a complexity of CYP gene expression in the field, and SsCYP4V cannot be used as a biomarker for the field monitoring of PAH pollution.
WOS关键词	POLYCYCLIC AROMATIC-HYDROCARBONS ; CYTOCHROME-P450 GENES ; PERINEREIS-NUNTIA ; NEREIS-VIRENS ; EXPRESSION ; BIOACCUMULATION ; SEDIMENT ; P450
WOS研究方向	Environmental Sciences & Ecology ; Water Resources
语种	英语
WOS记录号	WOS:000881525400001
资助机构	National Natural Science Foundation of China ; National Marine Public Welfare Research Project ; Young Marine Science Foundation of SOA
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/32404]
专题	海岸带生物学与生物资源利用重点实验室
通讯作者	Wang, Lili
作者单位	1.Minist Ecol & Environm, Natl Marine Environm Monitoring Ctr, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Yu, Zhenglin,Qi, Aimin,Wang, Lili,et al. A Novel CYP4 Gene Identified in the Polychaete Sternaspis scutata and Its Transcriptional Levels along the Coasts of the Liaodong Peninsula[J]. WATER,2022,14(21):12.
APA	Yu, Zhenglin.,Qi, Aimin.,Wang, Lili.,Shan, Encui.,Li, Dongmei.,...&Zhang, Anguo.(2022).A Novel CYP4 Gene Identified in the Polychaete Sternaspis scutata and Its Transcriptional Levels along the Coasts of the Liaodong Peninsula.WATER,14(21),12.
MLA	Yu, Zhenglin,et al."A Novel CYP4 Gene Identified in the Polychaete Sternaspis scutata and Its Transcriptional Levels along the Coasts of the Liaodong Peninsula".WATER 14.21(2022):12.