Transcriptome analysis of hepatopancreas and gills of Palaemon gravieri under salinity stress

doi:10.1016/j.gene.2022.147013

	Transcriptome analysis of hepatopancreas and gills of Palaemon gravieri under salinity stress
	Shi, Wenjun 3,5; Hu, Runhao 4,5; Zhao, Ran 4,5; Zhu, Jianqiang 4,5; Shen, Hui 5; Li, Hui 5; Wang, Libao 5; Yang, Zeyu 2; Jiang, Qi 5; Qiao, Yi 5
刊名	GENE
	2023-01-30
卷号	851 页码:15
关键词	Palaemon gravieri Transcriptome Salinity stress Metabolism Transporters
ISSN号	0378-1119
DOI	10.1016/j.gene.2022.147013
通讯作者	Wan, Xihe(wxh1708@163.com)
英文摘要	Salinity is an important factor in the aquatic environment, and its fluctuations always result in osmotic stress, which affects the survival, distribution, and physiological activities of crustaceans. Crustaceans counter them through osmoregulation, which consists of many mechanisms. Palaemon gravieri is an important economic spe-cies in Palaemonidae, widely distributed in the southern East China Sea and the China Yellow Sea, and has a good adaptability to salinity stress. Currently, there are only a few studies on the effects of salinity on P. graviera. Therefore, it is particularly important to study the molecular responses of P. gravieri to salinity fluctuations. In this study, P. gravieri was treated with salinities of 10, 25, and 40, and the hepatopancreas and gills of shrimp in the different salinity groups were sampled after 24 h. The samples were used for RNA extraction and tran-scriptome analysis. In total, 80,994 unigenes were obtained, of which 19,114 were annotated. The differences in gene expression between different tissues at the same salinity were more significant. Many metabolism-related genes were downregulated in the gills, such as beta-hexosaminidase subunit alpha (HEXA), 10-formyltetrahydro-folate dehydrogenase (ALDH1L1), and Alcohol dehydrogenase class-3 (ADH5). Scanning transmission electron microscope analysis showed that the expression levels of some stress-(but not salinity stress) related genes changed after stress (mostly upregulated), suggesting the existence of secondary stress. Gene set enrichment analysis (GSEA) focused on the expression of transporters in osmoregulation, and the results showed that they mainly played a role in the gills, but ATP-binding cassette (ABC) transporters were more active in the hepato-pancreas. This study showed that the response of P. gravieri to salinity change was different not only between the hepatopancreas and gills, but also between low salinity and higher salinity, and the ion transport-related genes were mainly expressed in the gills. Overall, these results improve our understanding of salt tolerance mechanism in P. gravieri.
WOS关键词	HIGH-DENSITY-LIPOPROTEIN ; PACIFIC WHITE SHRIMP ; H+-ATPASE ; LITOPENAEUS-VANNAMEI ; OSMOREGULATORY CAPACITY ; ALCOHOL-DEHYDROGENASE ; MOLECULAR-CLONING ; QUANTITATIVE PCR ; ANION CHANNELS ; NA+/K+-ATPASE
WOS研究方向	Genetics & Heredity
语种	英语
WOS记录号	WOS:000883040500005
资助机构	Jiangsu Provincial Agricultural Major New Varieties Creation Project ; Jiangsu Agricultural Industry Technology System ; Project of Seed Industry Revitalization in Jiangsu Province ; Jiangsu Agricultural Science and Technology Innovation Fund
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/32373]
专题	中国科学院烟台海岸带研究所
通讯作者	Wan, Xihe
作者单位	1.Inst Oceanol & Marine Fisheries, 31 Jiaoyu Rd, Nantong 226007, Jiangsu, Peoples R China 2.Aquat Prod Technol Extens Stn Gaoyou, Gaoyou 225634, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 4.Shanghai Ocean Univ, Natl Demonstrat Ctr Expt Fisheries Sci Educ, Shanghai 201306, Peoples R China 5.Inst Oceanol & Marine Fisheries, Nantong 226007, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Shi, Wenjun,Hu, Runhao,Zhao, Ran,et al. Transcriptome analysis of hepatopancreas and gills of Palaemon gravieri under salinity stress[J]. GENE,2023,851:15.
APA	Shi, Wenjun.,Hu, Runhao.,Zhao, Ran.,Zhu, Jianqiang.,Shen, Hui.,...&Wan, Xihe.(2023).Transcriptome analysis of hepatopancreas and gills of Palaemon gravieri under salinity stress.GENE,851,15.
MLA	Shi, Wenjun,et al."Transcriptome analysis of hepatopancreas and gills of Palaemon gravieri under salinity stress".GENE 851(2023):15.