Double-layer focal plane microscopy for high throughput DNA sequencing

doi:10.1364/oe.454100

CORC > 长春光学精密机械与物理研究所 > 中国科学院长春光学精密机械与物理研究所

	Double-layer focal plane microscopy for high throughput DNA sequencing
	X. Zhang; K. Kang; Y. F. Jiang; J. X. He and Y. F. Qiao
刊名	Optics Express
	2022
卷号	30 期号:11 页码:18496-18504
ISSN号	1094-4087
DOI	10.1364/oe.454100
英文摘要	Throughput is one of the most important properties in DNA sequencing. We propose a novel double-layer focal plane microscopy that doubles the DNA sequencing throughput. Each fluorescence channel is divided into two tube lens channels by energy splitting, and the camera is adjusted to take images corresponding to different defocus positions of the objective, thus doubling the information capacity of the microscopy. The microscopy is applied to gene chip, which has high spatial frequency and good uniformity, so the simultaneous imaging of the two tubes has little influence on each other due to the spatial averaging effect. Experimental results show that the image signal to noise ratio (SNR) is reduced by 1%, while the sequencing throughput is doubled. (C) 2022 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement
URL标识	查看原文
语种	英语
内容类型	期刊论文
源URL	[http://ir.ciomp.ac.cn/handle/181722/66574]
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	X. Zhang,K. Kang,Y. F. Jiang,et al. Double-layer focal plane microscopy for high throughput DNA sequencing[J]. Optics Express,2022,30(11):18496-18504.
APA	X. Zhang,K. Kang,Y. F. Jiang,&J. X. He and Y. F. Qiao.(2022).Double-layer focal plane microscopy for high throughput DNA sequencing.Optics Express,30(11),18496-18504.
MLA	X. Zhang,et al."Double-layer focal plane microscopy for high throughput DNA sequencing".Optics Express 30.11(2022):18496-18504.