Overexpression of adenosine 5'-monophosphate deaminase increased umami substance-Inosine 5'- mononucleotide and promoted Neopyropia yezoensis quality

doi:10.1016/j.algal.2022.102737

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Overexpression of adenosine 5'-monophosphate deaminase increased umami substance-Inosine 5'- mononucleotide and promoted Neopyropia yezoensis quality
	He, Bangxiang 2; Zheng, Zhenbing 2; Xie, Xiujun 2; Wang, Guangce 1,2
刊名	ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
	2022-06-01
卷号	65 页码:6
关键词	Adenosine-5'-monophosphate deaminase Neopyropia yezoensis Umami substance Genetic transformation
ISSN号	2211-9264
DOI	10.1016/j.algal.2022.102737
通讯作者	Xie, Xiujun(xjxie@qdio.ac.cn) ; Wang, Guangce(gcwang@qdio.ac.cn)
英文摘要	Precision molecular breeding technology plays an important role in improving the yield and quality of cultivated marine crops. In this study, we aimed to improve the quality of Neopyropia yezoensis, one of the important economic seaweeds. We constructed overexpression (OE)-, co-suppression (Ci)-, and RNAi (Ri)-type mutants of Nyampd (AMP deaminase of N. yezoensis), which could catalyze the decomposition of AMP into the umami substance inosine 5'- mononucleotide (IMP) through the genetic transformation system of N. yezoensis. Our results showed that the IMP content and growth rate of OE strain were significantly higher than those of wild type. In addition, in knockdown strains (Ci/Ri), a phenomenon similar to genetic compensation in knockout research occurred, i.e., the expression of Nyampd2 (isozyme of Nyampd) was significantly upregulated in Ri/Ci strains, and the IMP content increased in Ci/Ri strains. These results provide important knowledge for precise molecular breeding and genetic transformation research of N. yezoensis.
资助项目	National Key Research and Development Program of China[2018YFD0901500] ; National Natural Science Foundation of China[41906105] ; National Natural Science Foundation of China[41876163] ; Research Fund for the Taishan Scholar Project of Shandong Province[tspd20210316] ; Key Deployment Project of the Centre for Ocean Mega-Research of Science[COMS2019Q02] ; Major Scientific and Technological Innovation Projects in Shandong Province[2019JZZY010815] ; China Agriculture Research System of MOF and MARA[CARS-50] ; Qingdao Postdoctoral Funding[Y9KY161]
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000856083100010
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/180807]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Xie, Xiujun; Wang, Guangce
作者单位	1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	He, Bangxiang,Zheng, Zhenbing,Xie, Xiujun,et al. Overexpression of adenosine 5'-monophosphate deaminase increased umami substance-Inosine 5'- mononucleotide and promoted Neopyropia yezoensis quality[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2022,65:6.
APA	He, Bangxiang,Zheng, Zhenbing,Xie, Xiujun,&Wang, Guangce.(2022).Overexpression of adenosine 5'-monophosphate deaminase increased umami substance-Inosine 5'- mononucleotide and promoted Neopyropia yezoensis quality.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,65,6.
MLA	He, Bangxiang,et al."Overexpression of adenosine 5'-monophosphate deaminase increased umami substance-Inosine 5'- mononucleotide and promoted Neopyropia yezoensis quality".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 65(2022):6.