Temperature induces metabolic reprogramming in fish during bacterial infection

doi:10.3389/fimmu.2022.1010948

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Temperature induces metabolic reprogramming in fish during bacterial infection
	Sun, Bin 4,5; Sun, Boguang 3,5; Zhang, Beibei 2,5; Sun, Li 1,3,5
刊名	FRONTIERS IN IMMUNOLOGY
	2022-09-15
卷号	13 页码:16
关键词	Paralichthys olivaceus metabolism edwardsiellosis temperature global warming biomarker Edwardsiella bacterial infection
ISSN号	1664-3224
DOI	10.3389/fimmu.2022.1010948
通讯作者	Sun, Boguang() ; Sun, Li(lsun@qdio.ac.cn)
英文摘要	Water temperature elevation as a consequence of global warming results in increased incidence of bacterial disease, such as edwardsiellosis, in fish farming. Edwardsiellosis is caused by the bacterial pathogen Edwardsiella tarda and affects many farmed fish including flounder (Paralichthys olivaceus). Currently, the effect of temperature on the metabolic response of flounder to E. tarda infection is unclear. In this study, we found that compared to low temperature (15 degrees C), high temperature (23 degrees C) enhanced E. tarda dissemination in flounder tissues. To examine the impact of temperature on the metabolism of flounder induced by E. tarda, comparative metabolomics were performed, which identified a large number of metabolites responsive to E. tarda invasion and temperature alteration. During E. tarda infection, the metabolic profile induced by elevated temperature was mainly featured by extensively decreased amino acids and TCA intermediates such as succinate, a proven immune regulator. Further, 38 potential metabolite markers of temperature effect (MMTE) in association with bacterial infection were identified. When used as exogenous supplements, two of the MMTE, i.e., L-methionine and UDP-glucose, effectively upregulated the expression of pro-inflammatory cytokines and suppressed E. tarda infection in flounder leukocytes. Taken together, the results of this study indicate an important influence of temperature on the metabolism of flounder during bacterial infection, which eventually affects the survivability of the fish.
资助项目	National Key R&D Program of China ; National Natural Science Foundation of China ; Taishan Scholar Program of Shandong Province ; [2018YFD0900500] ; [41876170]
WOS研究方向	Immunology
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000862097800001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/180711]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Sun, Boguang; Sun, Li
作者单位	1.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing, Peoples R China 2.Qingdao Agr Univ, Coll Anim Sci & Technol, Qingdao, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Fujian Polytech Normal Univ, Inst Ocean Res, Fuqing, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS Key Lab Expt Marine Biol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Sun, Bin,Sun, Boguang,Zhang, Beibei,et al. Temperature induces metabolic reprogramming in fish during bacterial infection[J]. FRONTIERS IN IMMUNOLOGY,2022,13:16.
APA	Sun, Bin,Sun, Boguang,Zhang, Beibei,&Sun, Li.(2022).Temperature induces metabolic reprogramming in fish during bacterial infection.FRONTIERS IN IMMUNOLOGY,13,16.
MLA	Sun, Bin,et al."Temperature induces metabolic reprogramming in fish during bacterial infection".FRONTIERS IN IMMUNOLOGY 13(2022):16.