Two Foreign Antimicrobial Peptides Expressed in the Chloroplast of Porphyridium purpureum Possessed Antibacterial Properties

doi:10.3390/md20080484

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室

	Two Foreign Antimicrobial Peptides Expressed in the Chloroplast of Porphyridium purpureum Possessed Antibacterial Properties
	Han, Subing 2,3; Zhao, Jialin 1; Liu, Ping 3; Wang, Kang 2; Qin, Song 2; Zhao, Zhenjun 3; Cui, Yulin 2
刊名	MARINE DRUGS
	2022-08-01
卷号	20 期号:8 页码:11
关键词	Porphyridium purpureum chloroplast transformation system microparticle bombardment antimicrobial peptides NZ2114 Piscidin-4 inhibitory effect
DOI	10.3390/md20080484
英文摘要	To solve the problem of antibiotic abuse in aquaculture and to utilize the application potential of antimicrobial peptides (AMPs), a chloroplast transformation system of Porphyridium purpureum was successfully constructed for effectively expressing two exogenous AMPs. The endogenous fragments of 16S rDNA/trnA-23S rDNA were used as flanking fragments for the homologous recombination in the chloroplast genome. Two AMPs encoded by the transformation vector were controlled by the native promoter psbB in a polycistron. The plasmids were transferred into P. purpureum via particle bombardment and the transformation vectors were screened using phosphinothricin (bar), a dominant selection marker under the control of the psbA promoter. Subsequently, in the positive transformed colonies, the exogenous fragments were found to be inserted in the flanking fragments directionally as expected and two foreign AMPs were successfully obtained. Finally, two exogenous peptides with antibacterial properties were obtained from the transformed strain. The two AMPs expressed by the transformed strain were shown to have similar inhibitory effects to antibiotics by inhibition tests. This suggested that AMPs can be introduced into aquaculture using baited microalgae, providing new ideas and ways to solve a series of aquaculture diseases caused by bacteria.
资助项目	National Natural Science Foundation of China[42176140] ; National Natural Science Foundation of China[41876188] ; Project of Innovation and Development of Marine Economy[HHCL201803]
WOS关键词	TETRASELMIS SUBCORDIFORMIS PRASINOPHYCEAE ; PLECTASIN ; NZ2114 ; TRANSFORMATION ; CHLOROPHYTA ; VACCINES ; PROTEIN
WOS研究方向	Pharmacology & Pharmacy
语种	英语
出版者	MDPI
WOS记录号	WOS:000846719300001
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/31663]
专题	海岸带生物学与生物资源利用重点实验室
通讯作者	Cui, Yulin
作者单位	1.Yantai Marine Econ Res Inst, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Peoples R China 3.Yantai Univ, Coll Life Sci, Yantai 264000, Peoples R China
推荐引用方式 GB/T 7714	Han, Subing,Zhao, Jialin,Liu, Ping,et al. Two Foreign Antimicrobial Peptides Expressed in the Chloroplast of Porphyridium purpureum Possessed Antibacterial Properties[J]. MARINE DRUGS,2022,20(8):11.
APA	Han, Subing.,Zhao, Jialin.,Liu, Ping.,Wang, Kang.,Qin, Song.,...&Cui, Yulin.(2022).Two Foreign Antimicrobial Peptides Expressed in the Chloroplast of Porphyridium purpureum Possessed Antibacterial Properties.MARINE DRUGS,20(8),11.
MLA	Han, Subing,et al."Two Foreign Antimicrobial Peptides Expressed in the Chloroplast of Porphyridium purpureum Possessed Antibacterial Properties".MARINE DRUGS 20.8(2022):11.