Toxicological effects of tris(1,3-dichloro-2-propyl) phosphate in oyster Crassostrea gigas using proteomic and phosphoproteomic analyses

doi:10.1016/j.jhazmat.2022.128824

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	Toxicological effects of tris(1,3-dichloro-2-propyl) phosphate in oyster Crassostrea gigas using proteomic and phosphoproteomic analyses
	Yin, Chengcheng 3,5; Sun, Zuodeng 2; Ji, Chenglong 1,4,5; Li, Fei 1,5; Wu, Huifeng 1,4,5
刊名	JOURNAL OF HAZARDOUS MATERIALS
	2022-07-15
卷号	434 页码:12
关键词	Tris(1,3-dichloro-2-propyl) phosphate Crassostrea gigas Proteomics Phosphoproteomics
ISSN号	0304-3894
DOI	10.1016/j.jhazmat.2022.128824
英文摘要	As a typical organophosphorus pollutant, tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) has been widely detected in aquatic environment. Previous studies showed that protein phosphorylation might be a vital way of TDCIPP to exert multiple toxic effects. However, there is a lack of high- throughput investigations on how TDCIPP affected protein phosphorylation. In this study, the toxicological effects of TDCIPP were explored by proteomic and phosphoproteomic analyses together with traditional means in oysters Crassostrea gigas treated with 0.5, 5 and 50 mu g/L TDCIPP for 28 days. Integration of omic analyses revealed that TDCIPP dysregulated transcription, energy metabolism, and apoptosis and cell proliferation by either directly phosphorylating pivotal proteins or phosphorylating their upstream signaling pathways. The U-shaped response of acetylcholinesterase activities suggested the neurotoxicity of TDCIPP in a hormesis manner. What's more, the increase in caspase-9 activity as well as the expression or phosphorylation alterations in eukaryotic translation initiation factor 4E, cell division control protein 42 and transforming growth factor-beta 1-induced protein indicated the disruption of homeostasis between apoptosis and cell proliferation, which was consistent with the observation of shedding of digestive cells. Overall, combination of proteomic and phosphoproteomic analyses showed the capability of identifying molecular events, which provided new insights into the toxicological mechanisms of TDCIPP.
资助项目	National Natural Science Foundation of China[41976152] ; Yantai Science and Technology Development Plan[2020MSGY060]
WOS关键词	ORGANOPHOSPHATE FLAME RETARDANTS ; QUANTITATIVE ALTERATIONS ; PYRUVATE-DEHYDROGENASE ; PROSTATE-CANCER ; CELL ; PHOSPHORYLATION ; PROTEIN ; GROWTH ; BIOCONCENTRATION ; IDENTIFICATION
WOS研究方向	Engineering ; Environmental Sciences & Ecology
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000877072800002
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/31846]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者	Ji, Chenglong; Wu, Huifeng
作者单位	1.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 2.Shandong Fisheries Dev & Resource Conservat Ctr, Jinan 250013, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Fisheries Sci & Food Prod Processes, Qingdao 266237, Peoples R China 5.Chinese Acad Sci, Shandong Key Lab Coastal Environm Processes, CAS Key Lab Coastal Environm Processes & Ecol Rem, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai, Peoples R China
推荐引用方式 GB/T 7714	Yin, Chengcheng,Sun, Zuodeng,Ji, Chenglong,et al. Toxicological effects of tris(1,3-dichloro-2-propyl) phosphate in oyster Crassostrea gigas using proteomic and phosphoproteomic analyses[J]. JOURNAL OF HAZARDOUS MATERIALS,2022,434:12.
APA	Yin, Chengcheng,Sun, Zuodeng,Ji, Chenglong,Li, Fei,&Wu, Huifeng.(2022).Toxicological effects of tris(1,3-dichloro-2-propyl) phosphate in oyster Crassostrea gigas using proteomic and phosphoproteomic analyses.JOURNAL OF HAZARDOUS MATERIALS,434,12.
MLA	Yin, Chengcheng,et al."Toxicological effects of tris(1,3-dichloro-2-propyl) phosphate in oyster Crassostrea gigas using proteomic and phosphoproteomic analyses".JOURNAL OF HAZARDOUS MATERIALS 434(2022):12.