Molecular fluorescent probes for imaging and evaluation of peroxynitrite fluctuations in living cells and in vivo under hypoxic stress

doi:10.1016/j.snb.2022.132410

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	Molecular fluorescent probes for imaging and evaluation of peroxynitrite fluctuations in living cells and in vivo under hypoxic stress
	Huang, Yan 5; Yu, Lei 4; Fu, Lili 5; Hou, Junjun 2; Wang, Lingxiao 5; Sun, Mingzhao 5; Wang, Xiaoyan 3,5; Chen, Lingxin 1,3,5
刊名	SENSORS AND ACTUATORS B-CHEMICAL
	2022-11-01
卷号	370 页码:7
关键词	Fluorescent probe Peroxynitrite Hypoxia stress Near infrared Fluorescence imaging
DOI	10.1016/j.snb.2022.132410
英文摘要	Hypoxia stress causes cell damage and apoptosis by producing excessive reactive oxygen species (ROS), which is a universal concern in biology and medicine. Peroxynitrite (ONOO-) plays a vital part in the oxidative injury of tissue proteins in the pathogenic mechanism of many disorders, which is a one of ROS. Thus, excessive pro-duction of ONOO- may be one of the crucial factors leading to hypoxia stress injury. However, due to the unstable chemical property and strong reactivity of ONOO-, most of the existing detection ways cannot image and detect its level in real time. Herein, in order to selectively imaging and detecting ONOO- in living cells and in vivo, we synthesized a fluorescent probe Cy-CF3 (our fluorescent probe for detection of ONOO-). Cy-CF3 was capable of monitoring ONOO- with excellent sensitivity and selectivity. Using Cy-CF3, the relationship between hypoxia stress and ONOO- was evaluated. The probe was also employed to sense the change of ONOO- in zebrafish under hypoxia conditions as well as determine ONOO- in living mice with acute liver ischemia. Taken together, the proposed probe maybe a powerful chemical tool for the study of ONOO- in biological mechanisms.
资助项目	National Nature Science Foundation of China[21976209] ; National Nature Science Foundation of China[22007005] ; science and technology innovation development plan of Yantai of China[2020MSGY113] ; science and technology innovation development plan of Yantai of China[2020YT06000898] ; Research Initiation Fund of Binzhou Medical University[BY2019KYQD39] ; Research Initiation Fund of Binzhou Medical University[BY2020KYQD01] ; Taishan Scholar Project Special Funding[ts20190962] ; Shandong Peninsula Engineering Research Center of Comprehensive Brine Utilization[2018LS014] ; Weifang Science and technology development project[2019GX076]
WOS关键词	REACTIVE OXYGEN ; MITOCHONDRIA ; 2-PHOTON ; CONSTRUCTION ; DISEASE ; DNA
WOS研究方向	Chemistry ; Electrochemistry ; Instruments & Instrumentation
语种	英语
出版者	ELSEVIER SCIENCE SA
WOS记录号	WOS:000841960100004
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/31630]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者	Chen, Lingxin
作者单位	1.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 2.Binzhou Med Univ Hosp, Dept Resp Med, Binzhou 256603, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc,YICCAS, Yantai 264003, Peoples R China 4.Weifang Univ Sci & Technol, Shandong Peninsula Engn Res Ctr Comprehens Brine U, Weifang 262700, Peoples R China 5.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Huang, Yan,Yu, Lei,Fu, Lili,et al. Molecular fluorescent probes for imaging and evaluation of peroxynitrite fluctuations in living cells and in vivo under hypoxic stress[J]. SENSORS AND ACTUATORS B-CHEMICAL,2022,370:7.
APA	Huang, Yan.,Yu, Lei.,Fu, Lili.,Hou, Junjun.,Wang, Lingxiao.,...&Chen, Lingxin.(2022).Molecular fluorescent probes for imaging and evaluation of peroxynitrite fluctuations in living cells and in vivo under hypoxic stress.SENSORS AND ACTUATORS B-CHEMICAL,370,7.
MLA	Huang, Yan,et al."Molecular fluorescent probes for imaging and evaluation of peroxynitrite fluctuations in living cells and in vivo under hypoxic stress".SENSORS AND ACTUATORS B-CHEMICAL 370(2022):7.