Single-Cell Transcriptome Analysis of Radiation Pneumonitis Mice

doi:10.3390/antiox11081457

	Single-Cell Transcriptome Analysis of Radiation Pneumonitis Mice
	Yang, Miaomiao 2,3,4; Fan, Qiang 2,3; Hei, Tom K.5; Chen, Guodong 2; Cao, Wei 2,3; Meng, Gang 4; Han, Wei 1,2,6
刊名	ANTIOXIDANTS
	2022-08-01
卷号	11
关键词	radiation pneumonitis (RP) single-cell RNA-seq (scRNA-seq) mouse oxidative stress
DOI	10.3390/antiox11081457
通讯作者	Han, Wei(hanw@hfcas.ac.cn)
英文摘要	Radiation-induced lung injury (RILI), especially radiation pneumonitis (RP), is a common clinical complication associated with thoracic radiotherapy for malignant tumors. However, the specific contributions of each cell subtype to this process are unknown. Here, we provide the single-cell pathology landscape of the RP in a mouse model by unbiased single-cell RNA-seq (scRNA-seq). We found a decline of type 2 alveolar cells in the RP lung tissue, with an expansion of macrophages, especially the Fabp4low and Spp1high subgroup, while Fabp4high macrophages were almost depleted. We observed an elevated expression of multiple mitochondrial genes in the RP group, indicating a type 2 alveolar cell (AT2) response to oxidative stress. We also calculated the enrichment of a cGAS-STING signaling pathway, which may be involved in regulating inflammatory responses and cancer progression in AT2 cells of PR mice. We delineate markers and transcriptional states, identify a type 2 alveolar cell, and uncover fundamental determinants of lung fibrosis and inflammatory response in RP lung tissue of mice.
资助项目	National Natural Science Foundation of China[81974484] ; National Natural Science Foundation of China[U20A20372] ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology[2019HSC-CIP010] ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection
WOS关键词	LUNG ENDOTHELIAL-CELLS ; PULMONARY-FIBROSIS ; INJURY ; EXPRESSION ; CANCER
WOS研究方向	Biochemistry & Molecular Biology ; Pharmacology & Pharmacy ; Food Science & Technology
语种	英语
出版者	MDPI
WOS记录号	WOS:000846315000001
资助机构	National Natural Science Foundation of China ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/131814]
专题	中国科学院合肥物质科学研究院
通讯作者	Han, Wei
作者单位	1.Soochow Univ, Sch Radiol & Interdisciplinary Sci RAD X, Suzhou 215123, Peoples R China 2.Chinese Acad Sci, Hefei Inst Phys Sci, Anhui Prov Key Lab Med Phys & Technol, Inst Hlth & Med Technol, Hefei 230031, Peoples R China 3.Univ Sci & Technol China, Sci Isl Branch, Grad Sch, Hefei 230026, Peoples R China 4.Anhui Med Univ, Clin Pathol Ctr, Affiliated Hosp 4, Hefei 230022, Peoples R China 5.Columbia Univ, Coll Phys & Surg, Ctr Radiol Res, Med Ctr, New York, NY 10032 USA 6.Soochow Univ, Collaborat Innovat Ctr Radiat Med Jiangsu Higher, Suzhou 215123, Peoples R China
推荐引用方式 GB/T 7714	Yang, Miaomiao,Fan, Qiang,Hei, Tom K.,et al. Single-Cell Transcriptome Analysis of Radiation Pneumonitis Mice[J]. ANTIOXIDANTS,2022,11.
APA	Yang, Miaomiao.,Fan, Qiang.,Hei, Tom K..,Chen, Guodong.,Cao, Wei.,...&Han, Wei.(2022).Single-Cell Transcriptome Analysis of Radiation Pneumonitis Mice.ANTIOXIDANTS,11.
MLA	Yang, Miaomiao,et al."Single-Cell Transcriptome Analysis of Radiation Pneumonitis Mice".ANTIOXIDANTS 11(2022).