Novel carboxymethyl chitosan/N-acetylneuraminic acid hydrogel for the protection of Pediococcus pentosaceus

doi:10.1016/j.foodres.2022.111355

	Novel carboxymethyl chitosan/N-acetylneuraminic acid hydrogel for the protection of Pediococcus pentosaceus
	Ge, Yuanfei 1,2; Wu, Jinyong 2; Pang, Min 3; Hu, Dan 1,2; Li, Zhongkui 2; Wang, Xiangqin 2; Sun, Lijie 2; Chen, Xiangsong 2; Yao, Jianming 1,2
刊名	FOOD RESEARCH INTERNATIONAL
	2022-06-01
卷号	156
关键词	Carboxymethyl chitosan N-acetylneuraminic acid Hydrogel Encapsulation Pediococcus pentosaceus
ISSN号	0963-9969
DOI	10.1016/j.foodres.2022.111355
通讯作者	Chen, Xiangsong(xschen@ipp.cas.cn) ; Yao, Jianming(jmyaocas@163.com)
英文摘要	In this study, carboxymethyl chitosan (CMCS) and N-acetylneuraminic acid (NeuAc) were used to develop CNeuAc hydrogels to encapsulate Pediococcus pentosaceus QK-1. The mechanical properties, thermal stability, in vitro degradation, and pH sensitivity of the hydrogel were evaluated. The C-NeuAc concentration required for optimal hydrogel performance was 3% (w/v). Hydrogel swelling behaviour was effectively assessed by Fickian diffusion and Schott's second-order kinetic models. The hydrogel demonstrated excellent biocompatibility and low in vitro cytotoxicity. An in vitro assay revealed that the viability of Pediococcus pentosaceus QK-1 in C-NeuAc had decreased by only 1.41 log (CFU/ mL) after exposure to simulated acidic gastric fluid. Moreover, the survival rate of the encapsulated and free Pediococcus pentosaceus QK-1 cells were 80.1% and virtually zero, respectively, after passage through the gastrointestinal tract. It was empirically determined that low temperature and freezedrying were the ideal condition and method to ensure storage longevity of the hydrogel-encapsulated probiotic. Hence, C-NeuAc hydrogel is highly desirable as a food-grade probiotic delivery vehicle.
资助项目	postdoctoral research funding project of Anhui province[2020B440] ; Special Project of Science and Technology Cooperation between Hubei Province and Chinese Academy of Sciences[2021-01-09] ; Wuhan Science and Technology Plan Project Enterprise Technology Innovation Special Project[2020020602012119]
WOS关键词	NEURAMINIC ACID ; NANOCOMPOSITES ; ADSORPTION ; PH
WOS研究方向	Food Science & Technology
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000810383900011
资助机构	postdoctoral research funding project of Anhui province ; Special Project of Science and Technology Cooperation between Hubei Province and Chinese Academy of Sciences ; Wuhan Science and Technology Plan Project Enterprise Technology Innovation Special Project
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/131261]
专题	中国科学院合肥物质科学研究院
通讯作者	Chen, Xiangsong; Yao, Jianming
作者单位	1.Univ Sci & Technol China, Hefei 230026, Peoples R China 2.Chinese Acad Sci, Hefei Inst Phys Sci, Inst Plasma Phys, Hefei 230031, Peoples R China 3.Hefei Univ Technol, Sch Food & Biol Engn, Hefei 230601, Peoples R China
推荐引用方式 GB/T 7714	Ge, Yuanfei,Wu, Jinyong,Pang, Min,et al. Novel carboxymethyl chitosan/N-acetylneuraminic acid hydrogel for the protection of Pediococcus pentosaceus[J]. FOOD RESEARCH INTERNATIONAL,2022,156.
APA	Ge, Yuanfei.,Wu, Jinyong.,Pang, Min.,Hu, Dan.,Li, Zhongkui.,...&Yao, Jianming.(2022).Novel carboxymethyl chitosan/N-acetylneuraminic acid hydrogel for the protection of Pediococcus pentosaceus.FOOD RESEARCH INTERNATIONAL,156.
MLA	Ge, Yuanfei,et al."Novel carboxymethyl chitosan/N-acetylneuraminic acid hydrogel for the protection of Pediococcus pentosaceus".FOOD RESEARCH INTERNATIONAL 156(2022).