Identifying Heterozipper ?-Sheet in Twisted Amyloid Aggregation br

doi:10.1021/acs.nanolett.2c00596

	Identifying Heterozipper ?-Sheet in Twisted Amyloid Aggregation br
	Song, Yongxiu 6,7,8; Dai, Bin 1; Wang, Yong 2; Wang, Yin 7; Liu, Cong 5; Gourdon, Pontus 3,4; Liu, Lei 6; Wang, Kaituo 4; Dong, Mingdong 7
刊名	NANO LETTERS
	2022-05-11
卷号	22
关键词	peptide self-assembly atomic force microscopy cryo-electron microscopy (cryo-EM) hierarchical nanostructure twisted nanofibrils
ISSN号	1530-6984
DOI	10.1021/acs.nanolett.2c00596
通讯作者	Liu, Lei(liual@ujs.edu.cn) ; Wang, Kaituo(kaituo@sund.ku.dk) ; Dong, Mingdong(dong@inano.au.dk)
英文摘要	Amyloid peptide (AP) self-assembly is a hierarch-ical process. However, the mechanistic rule of guiding peptides toorganize well-ordered nanostructure in a clear and precise mannerremains poorly understood. Herein we explored the molecularinsight of AP motif aggregates underlying hierarchical process withhelicalfibrillar structure by atomic force microscope, cryo-electronmicroscopy (cryo-EM), and molecular dynamics simulation. APassembly encompasses well-ordered twistedfibrils with uniformmorphology, size, and periodicity. More importantly, a hetero-zipper beta-sheet was identified in a protofilament of AP assemblydetermined by cryo-EM with a high resolution of 3.5 A. Eachpeptide heterozipper was further composed of two antiparallel beta strands and arranged by an alternative manner in a protofilament.The hydrophobic core and hydrophilic area in each zipper played the significant role for peptide assembling. This work proposedand verified the rule facilitating the basic building unit to form twistedfibrils and gave the explanation of peptide hierarchicalassembling
资助项目	National Natural Science Foundation of China[22072060] ; National Natural Science Foundation of China[21573097] ; Independent Research Fund Denmark ; EU H2020RISE 2016-MNR4S Cell project ; Zhejiang Province Post-Doctoral Scientific Research Fund[269745]
WOS关键词	PROTEIN SECONDARY STRUCTURE ; DICHROWEB ; PEPTIDE
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000801231700028
资助机构	National Natural Science Foundation of China ; Independent Research Fund Denmark ; EU H2020RISE 2016-MNR4S Cell project ; Zhejiang Province Post-Doctoral Scientific Research Fund
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/131039]
专题	中国科学院合肥物质科学研究院
通讯作者	Liu, Lei; Wang, Kaituo; Dong, Mingdong
作者单位	1.Shanghai Jiao Tong Univ, Sch Sensing Sci & Engn, Shanghai 200240, Peoples R China 2.Zhejiang Univ, Coll Life Sci, Shanghai Inst Adv Study, Inst Quantitat Biol,Prov Int Sci & Technol Cooper, Int Campus, Haining 314400, Peoples R China 3.Lund Univ, Dept Expt Med Sci, S-22363 Lund, Sweden 4.Univ Copenhagen, Dept Biomed Sci, DK-2200 Copenhagen, Denmark 5.Chinese Acad Sci, Interdisciplinary Res Ctr Biol & Chem, Shanghai Inst Organ Chem, Shanghai 200032, Peoples R China 6.Jiangsu Univ, Inst Adv Mat, Zhenjiang 212013, Jiangsu, Peoples R China 7.Aarhus Univ, Interdisciplinary Nanosci Ctr iNANO, DK-8000 Aarhus, Denmark 8.Chinese Acad Sci, Univ Chinese Acad Sci, Inst Basic Med & Canc IBMC, Canc Hosp,Zhejiang Canc Hos, Hangzhou 310022, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Song, Yongxiu,Dai, Bin,Wang, Yong,et al. Identifying Heterozipper ?-Sheet in Twisted Amyloid Aggregation br[J]. NANO LETTERS,2022,22.
APA	Song, Yongxiu.,Dai, Bin.,Wang, Yong.,Wang, Yin.,Liu, Cong.,...&Dong, Mingdong.(2022).Identifying Heterozipper ?-Sheet in Twisted Amyloid Aggregation br.NANO LETTERS,22.
MLA	Song, Yongxiu,et al."Identifying Heterozipper ?-Sheet in Twisted Amyloid Aggregation br".NANO LETTERS 22(2022).