A dynamic fluorescence probed glycolysis suppression process in HeLa cells treated with trichostatin A | |
Zhang, Fengqiu1,2; Shao, Changsheng1,3; Wu, Yahui1,3; Zhao, Wei2; Jing, Xumiao2; Fang, Cao1,3; Huang, Qing1,3 | |
刊名 | ANALYST
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2022-09-21 | |
ISSN号 | 0003-2654 |
DOI | 10.1039/d2an01114j |
通讯作者 | Huang, Qing(huangq@ipp.ac.cn) |
英文摘要 | Acetylation can be regulated by histone deacetylases (HDACs) and histone acetyltransferases (HATs), and the imbalance between HDACs and HATs can lead to cancers. Trichostatin A (TSA), as one of the typical HDAC inhibitors (HDACis), may regulate the acetylation level and thus prevent the proliferation of cancer cells, in which the metabolic change of glycolysis is involved. However, the dynamic process of glycolysis has not yet been explored, and the mechanism is still elusive. In this work, we constructed GFP-actin-HeLa cells to observe the dynamic change of glycolysis in situ and found that the GFP fluorescence enhanced significantly after TSA treatment, which was consistent with the change of pH in the cells (pH(i)) depending on the change of lactate originated from glycolysis. Furthermore, we confirmed that the glycolysis was inhibited after TSA treatment, and this process was associated with HIF-1 alpha and c-Myc regulation. As such, this work not only demonstrates the usefulness of the GFP fluorescent probe for monitoring the metabolic process of glycolysis in situ, but also sheds new light on the mechanism of glycolysis suppression in the cancer cells treated with HDACi. |
资助项目 | National Nature Science Foundation of China[11635013] ; College Key Research Project in Henan Province[22A180030] ; Research Integration Program of Hefei Institutes of Physical Science, Chinese Academy of Sciences |
WOS关键词 | HISTONE DEACETYLASE INHIBITORS ; AEROBIC GLYCOLYSIS ; GENE ; ACETYLATION ; METABOLISM ; LACTATE ; PROTEIN ; PH ; HIF-1-ALPHA ; EXPRESSION |
WOS研究方向 | Chemistry |
语种 | 英语 |
出版者 | ROYAL SOC CHEMISTRY |
WOS记录号 | WOS:000870356500001 |
资助机构 | National Nature Science Foundation of China ; College Key Research Project in Henan Province ; Research Integration Program of Hefei Institutes of Physical Science, Chinese Academy of Sciences |
内容类型 | 期刊论文 |
源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/129444] ![]() |
专题 | 中国科学院合肥物质科学研究院 |
通讯作者 | Huang, Qing |
作者单位 | 1.Chinese Acad Sci, Inst Intelligent Machines, Hefei Inst Phys Sci, CAS Key Lab High Magnet Field & Ion Beam Phys Bio, Hefei, Peoples R China 2.Zhengzhou Univ, Sch Phys & Microelect, Henan Key Lab Ion Beam Bioengn, Zhengzhou, Peoples R China 3.Univ Sci & Technol China, Grad Sch, Sci Isl Branch, Hefei, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, Fengqiu,Shao, Changsheng,Wu, Yahui,et al. A dynamic fluorescence probed glycolysis suppression process in HeLa cells treated with trichostatin A[J]. ANALYST,2022. |
APA | Zhang, Fengqiu.,Shao, Changsheng.,Wu, Yahui.,Zhao, Wei.,Jing, Xumiao.,...&Huang, Qing.(2022).A dynamic fluorescence probed glycolysis suppression process in HeLa cells treated with trichostatin A.ANALYST. |
MLA | Zhang, Fengqiu,et al."A dynamic fluorescence probed glycolysis suppression process in HeLa cells treated with trichostatin A".ANALYST (2022). |
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