Preparative isolation of antioxidative furanocoumarins from Dracocephalum heterophyllum and their potential action target

	Preparative isolation of antioxidative furanocoumarins from Dracocephalum heterophyllum and their potential action target
	Lv, Y; Li, CZ; Wang, Z; Wang, QL; Li, G; Dang, J
刊名	JOURNAL OF SEPARATION SCIENCE
	2022
英文摘要	Traditional Tibetan medicine has extensively documented the health benefits of Dracocephalum heterophyllum. However, there are few reports on the chemical composition of furanocoumarins, probably because of their complicated isolation and purification procedures. In this study, four antioxidative furanocoumarins were isolated from Dracocephalum heterophyllum by medium- and high-pressure liquid chromatography in combination with on-line high-performance liquid chromatography-1,1-diphenyl-2-picrylhydrazyl recognition. Crude samples were sequentially pretreated by medium-pressure liquid chromatography using silica gel, MCI GEL CHP20P, and diol as stationary phases, whereas on-line high-performance liquid chromatography-1,1-diphenyl-2-picrylhydrazyl system was used to recognize antioxidant peaks in target fractions. Thereafter, the antioxidative peaks were separated and purified through high-pressure liquid chromatography to obtain four furanocoumarins with purities greater than 95%; namely isodemethylfuropinarine, demethylfuropinarine, alloimperatorin, and alloisoimperatorin. Finally, the antioxidant capacity of the isolated furanocoumarins was determined using in vitro experiments (1,1-diphenyl-2-picrylhydrazyl assays, molecular docking, and cellular validation) and it was concluded that nuclear factor erythroid 2-related factor 2 protein is a potential target of these compounds for their antioxidation effects. Thus, the proposed methodology exhibits excellent efficacy for the preparative isolation of high-purity antioxidative furanocoumarins from extracts of Dracocephalum heterophyllum and it can be efficiently utilized for isolating antioxidants from other natural products.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/61074]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Lv, Y,Li, CZ,Wang, Z,et al. Preparative isolation of antioxidative furanocoumarins from Dracocephalum heterophyllum and their potential action target[J]. JOURNAL OF SEPARATION SCIENCE,2022.
APA	Lv, Y,Li, CZ,Wang, Z,Wang, QL,Li, G,&Dang, J.(2022).Preparative isolation of antioxidative furanocoumarins from Dracocephalum heterophyllum and their potential action target.JOURNAL OF SEPARATION SCIENCE.
MLA	Lv, Y,et al."Preparative isolation of antioxidative furanocoumarins from Dracocephalum heterophyllum and their potential action target".JOURNAL OF SEPARATION SCIENCE (2022).