Material properties of phase-separated TFEB condensates regulate the autophagy-lysosome pathway

doi:10.1083/jcb.202112024

CORC > 力学研究所 > 中国科学院力学研究所 > 非线性力学国家重点实验室

	Material properties of phase-separated TFEB condensates regulate the autophagy-lysosome pathway
	Wang,Zheng 1; Chen,Di 1,2; Guan DS(关东石)3; Liang,Xiaobo 2,4; Xue,Jianfeng 5; Zhao HY(赵宏宇)1; Song,Guangtao 2,4; Lou,Jizhong 2,4; He,Yan 5; Zhang,Hong 1,2
刊名	JOURNAL OF CELL BIOLOGY
	2022-03-16
卷号	221 期号:5 页码:27
ISSN号	0021-9525
DOI	10.1083/jcb.202112024
通讯作者	Zhang, Hong(hongzhang@ibp.ac.cn)
英文摘要	Very little is known about how the material properties of protein condensates assembled via liquid-liquid phase separation (LLPS) are maintained and affect physiological functions. Here we show that liquid-like condensates of the transcription factor TFEB exhibit low fusion propensity in vitro and in living cells. We directly measured the attraction force between droplets, and we characterized the interfacial tension, viscosity, and elasticity of TFEB condensates. TFEB condensates contain rigid interfacial boundaries that govern their interaction behaviors. Several small molecules, including Ro-3306, modify the material properties of TFEB condensates, increasing their size and fusion propensity. These compounds promote lysosomal biogenesis and function in a TFEB-dependent manner without changing its cytoplasmic-nuclear translocation. Ro-3306 promotes autophagy activity, facilitating degradation of toxic protein aggregates. Our study helps explain how protein condensates are maintained as physically separate entities and reveals that the material properties of TFEB condensates can be harnessed to modulate TFEB activity.
分类号	二类/Q1
资助项目	Chinese Ministry of Science and Technology[2017YFA0503401] ; Beijing Municipal Science and Technology Committee[Z181100001318003] ; National Natural Science Foundation of China[92054301] ; National Natural Science Foundation of China[31790403] ; National Natural Science Foundation of China[31871426] ; Key Research Program of Frontier Sciences from the Chinese Academy of Sciences[QYZDYSSW-SMC006]
WOS关键词	PROTEIN ; DROPLETS ; PHOSPHORYLATION ; DETERMINES ; MATURATION ; GRANULES ; DYNAMICS ; SYSTEM ; CELLS
WOS研究方向	Cell Biology
语种	英语
WOS记录号	WOS:000867730300001
资助机构	Chinese Ministry of Science and Technology ; Beijing Municipal Science and Technology Committee ; National Natural Science Foundation of China ; Key Research Program of Frontier Sciences from the Chinese Academy of Sciences
其他责任者	Zhang, Hong
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/90400]
专题	力学研究所_非线性力学国家重点实验室
作者单位	1.Chinese Acad Sci, CAS Ctr Excellence Biomacromol, Inst Biophys, Natl Lab Biomacromol, Beijing, Peoples R China; 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China; 3.Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, Beijing, Peoples R China; 4.Chinese Acad Sci, CAS Ctr Excellence Biomacromol, Inst Biophys, Key Lab RNA Biol, Beijing, Peoples R China; 5.Tsinghua Univ, Dept Chem, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Minist Educ, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wang,Zheng,Chen,Di,Guan DS,et al. Material properties of phase-separated TFEB condensates regulate the autophagy-lysosome pathway[J]. JOURNAL OF CELL BIOLOGY,2022,221(5):27.
APA	Wang,Zheng.,Chen,Di.,关东石.,Liang,Xiaobo.,Xue,Jianfeng.,...&Zhang,Hong.(2022).Material properties of phase-separated TFEB condensates regulate the autophagy-lysosome pathway.JOURNAL OF CELL BIOLOGY,221(5),27.
MLA	Wang,Zheng,et al."Material properties of phase-separated TFEB condensates regulate the autophagy-lysosome pathway".JOURNAL OF CELL BIOLOGY 221.5(2022):27.