Cystathionine beta synthase participates in murine oocyte maturatione mediated by homocysteine | |
Liang, Rong ; Yu, Wei-Dong ; Du, Jun-Bao ; Yang, Li-Jun ; Yang, Jing-Jing ; Xu, Jian ; Shang, Mei ; Guo, Jing-Zhu | |
刊名 | reproductive toxicology
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2007 | |
关键词 | RNA interference small-interfering (si) RNA oocyte development granulosa cells cystathionine beta synthase (CBS) homocysteine RNA INTERFERENCE MAMMALIAN OOCYTES MOUSE OOCYTES EXPRESSION GENE MICE ANEUPLOIDY DEFECTS EMBRYOS GROWTH |
DOI | 10.1016/j.reprotox.2007.04.002 |
英文摘要 | Our previous study demonstrated that cystathionine P synthase (CBS) is highly expressed in the cumulus-oocyte complex during ovulation. However, the role of CES during oocyte maturation remains uncertain. In this study, a small-interfering (si) RNA interference (siRNA) approach was used to investigate the potential role of CBS during oocyte maturation. Accompanied with a gradual increase of homocysteine, the introduction of CBS-siRNA into marine granulosa cells selectively depleted the corresponding target mRNA and protein for CBS as assessed by semi-quantitative reverse-transcriptase PCR (RT-PCR) and immuofluorescence staining. When fully grown, germinal vesicle (GV) oocytes matured in vitro for 16 h using medium from transfected granulosa cells, the functional suppression of CBS resulted in a significant increase in the rate of GV-arrested oocytes. The results of this study provide evidence that CBS participates in the process of oocyte maturation. Furthermore, this effect may be fulfilled by conditioning the level of homocysteine in the microenvironment of the oocyte. (c) 2007 Published by Elsevier Inc.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000248775200009&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Reproductive Biology; Toxicology; SCI(E); PubMed; 11; ARTICLE; 1,SI; 89-96; 24 |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.pku.edu.cn/handle/20.500.11897/198318] ![]() |
专题 | 历史学系 |
推荐引用方式 GB/T 7714 | Liang, Rong,Yu, Wei-Dong,Du, Jun-Bao,et al. Cystathionine beta synthase participates in murine oocyte maturatione mediated by homocysteine[J]. reproductive toxicology,2007. |
APA | Liang, Rong.,Yu, Wei-Dong.,Du, Jun-Bao.,Yang, Li-Jun.,Yang, Jing-Jing.,...&Guo, Jing-Zhu.(2007).Cystathionine beta synthase participates in murine oocyte maturatione mediated by homocysteine.reproductive toxicology. |
MLA | Liang, Rong,et al."Cystathionine beta synthase participates in murine oocyte maturatione mediated by homocysteine".reproductive toxicology (2007). |
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