AtHKT1;1 and AtHAK5 mediate low-affinity Na+ uptake in Arabidopsis thaliana under mild salt stress

doi:10.1007/s10725-014-9964-2

	AtHKT1;1 and AtHAK5 mediate low-affinity Na+ uptake in Arabidopsis thaliana under mild salt stress
	Wang, Qian 2; Guan, Chao 2; Wang, Pei 2; Lv, Mao-Lin 2; Ma, Qing 2; Wu, Guo-Qiang 1; Bao, Ai-Ke 2; Zhang, Jin-Lin 2; Wang, Suo-Min 2
刊名	PLANT GROWTH REGULATION
	2015-04
卷号	75 期号:3 页码:615-623
关键词	Mild salt stress AtHKT1 Low-affinity Na+ uptake 1 Na-22(+) influx Net Na+ uptake rate AtHAK5
ISSN号	0167-6903
DOI	10.1007/s10725-014-9964-2
英文摘要	Salinity is a serious problem for agricultural production worldwide. Reducing Na+ influx is one of the key steps for controlling Na+ accumulation in plants and improving salt tolerance of crop plants. Researches on a number of species are now converging on HKT-type and KUP/HAK/KT type proteins, both of them are probable candidates of Na+ uptake into the root. To assess the contribution of AtHKT1;1 and AtHAK5 to low-affinity Na+ uptake in Arabidopsis thaliana, the Na-22(+) influx in A. thaliana wild type (WT) and hkt1;1 mutant (athkt1;1) with or without inhibitors (10 mM TEA(+) or 5 mM NH4 (+)) were investigated, in addition, the expression levels of AtHKT1;1 and AtHAK5 in plants exposed to different concentrations of NaCl, KCl or KCl plus NaCl were analyzed. Results showed that TEA(+) or NH4 (+) have no significant influence on Na-22(+) influx in WT, but reduced Na-22(+) influx by 42 and 46 %, respectively, in athkt1;1. Under 25 mM NaCl, 0.01 mM K+ facilitated higher net Na+ uptake rate in both WT and athkt1;1 than 2.5 mM K+. In addition, 0.01 mM K+ down-regulated AtHKT1;1 and up-regulated AtHAK5 in WT roots compared with 2.5 mM K+, and more interestingly, the transcript of AtHAK5 in athkt1;1 roots was always higher than that in WT roots during 48 h of 2.5 mM K+ plus 25 mM NaCl, and it increased continuously during 48 h of 0.01 mM K+ plus 25 mM NaCl. Therefore, it is proposed that AtHKT1;1 and AtHAK5 mediate low-affinity Na+ uptake, and both of them are regulated by external K+ concentrations. AtHKT1;1 might mediate low-affinity Na+ uptake under 2.5 mM K+, while 0.01 mM K+ might activate AtHAK5 and facilitate low-affinity Na+ uptake in WT. When AtHKT1;1 lost its function, AtHAK5 might mediate low-affinity Na+ uptake instead of AtHKT1;1 and this function becomes more important under low K+ condition.
资助项目	Specialized Research Fund for the Doctoral Program of Higher Education of China[20130211130001]
WOS研究方向	Plant Sciences
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000351210700004
状态	已发表
内容类型	期刊论文
源URL	[http://119.78.100.223/handle/2XXMBERH/34085]
专题	生命科学与工程学院
通讯作者	Wang, Suo-Min
作者单位	1.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Peoples R China 2.Lanzhou Univ, Coll Pastoral Agr Sci & Technol, State Key Lab Grassland Agroecosyst, Lanzhou 730020, Peoples R China
推荐引用方式 GB/T 7714	Wang, Qian,Guan, Chao,Wang, Pei,et al. AtHKT1;1 and AtHAK5 mediate low-affinity Na+ uptake in Arabidopsis thaliana under mild salt stress[J]. PLANT GROWTH REGULATION,2015,75(3):615-623.
APA	Wang, Qian.,Guan, Chao.,Wang, Pei.,Lv, Mao-Lin.,Ma, Qing.,...&Wang, Suo-Min.(2015).AtHKT1;1 and AtHAK5 mediate low-affinity Na+ uptake in Arabidopsis thaliana under mild salt stress.PLANT GROWTH REGULATION,75(3),615-623.
MLA	Wang, Qian,et al."AtHKT1;1 and AtHAK5 mediate low-affinity Na+ uptake in Arabidopsis thaliana under mild salt stress".PLANT GROWTH REGULATION 75.3(2015):615-623.