Co-expression of xerophyte Zygophyllum xanthoxylum ZxNHX and ZxVP1-1 confers enhanced salinity tolerance in chimeric sugar beet (Beta vulgaris L.)

doi:10.3389/fpls.2015.00581

	Co-expression of xerophyte Zygophyllum xanthoxylum ZxNHX and ZxVP1-1 confers enhanced salinity tolerance in chimeric sugar beet (Beta vulgaris L.)
	Wu, Guo-Qiang 1; Feng, Rui-Jun 1; Wang, Suo-Min 3; Wang, Chun-Mei 2; Bao, Ai-Ke 3; Wei, Li 3; Yuan, Hui-Jun 1
刊名	FRONTIERS IN PLANT SCIENCE
	2015-07-28
卷号	6
关键词	salinity tolerance tonoplast Na+/H+ antiporter H+-PPase Na+ compartmentalization sugar beet
ISSN号	1664-462X
DOI	10.3389/fpls.2015.00581
英文摘要	Salinity is one of the major abiotic stresses that limit the growth and productivity of sugar beet (Beta vulgaris L.). To improve sugar beet's salinity tolerance, the ZxNHX and ZxVP1-1 genes encoding tonoplast Na+/H+ antiporter and H+-PPase from xerophyte Zygophyllum xanthoxylum were co-expressed by Agrobacterium tumefaciens-mediated transformation. It is showed here that co-expression of ZxNHX and ZxVP1-1 confers enhanced salinity tolerance to the transformed sugar beet plants compared with the wild-type (WT) plants. The chimeric plants grew well in the presence of high salinity (400 mM NaCl), whereas WT plants displayed chlorosis and died within 8 days. Compared to WT plants, the chimeric plants co-expressing ZxNHX and ZxVP1-1 accumulated more proline, Na+ and K+ in their leaves and petioles when exposed to high salinity, which caused lower solute potential, retained more water and thus subjected to lesser cell membrane damage. Interestingly, the chimeric plants accumulated higher sucrose, glucose and fructose contents in their storage roots than WT plants in the absence or presence of high salinity. Our results suggested that co-expression of ZxNHX and ZxVP1-1 improved the osmoregulatory capacity in chimeric sugar beet through increased compartmentalization of ions into the vacuoles by enhancing the activity of proton pumps and thus mitigated Na+-toxicity for plants.
资助项目	Elitist Program of Lanzhou University of Technology[J201404]
WOS研究方向	Plant Sciences
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000358725400001
状态	已发表
内容类型	期刊论文
源URL	[http://119.78.100.223/handle/2XXMBERH/34015]
专题	生命科学与工程学院
通讯作者	Wu, Guo-Qiang
作者单位	1.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Peoples R China 2.Chinese Acad Agr Sci, Lanzhou Inst Husb & Pharmaceut Sci, Lanzhou, Peoples R China 3.Lanzhou Univ Technol, State Key Lab Grassland Agroecosyst, Coll Pastoral Agr Sci & Technol, Lanzhou 730050, Peoples R China
推荐引用方式 GB/T 7714	Wu, Guo-Qiang,Feng, Rui-Jun,Wang, Suo-Min,et al. Co-expression of xerophyte Zygophyllum xanthoxylum ZxNHX and ZxVP1-1 confers enhanced salinity tolerance in chimeric sugar beet (Beta vulgaris L.)[J]. FRONTIERS IN PLANT SCIENCE,2015,6.
APA	Wu, Guo-Qiang.,Feng, Rui-Jun.,Wang, Suo-Min.,Wang, Chun-Mei.,Bao, Ai-Ke.,...&Yuan, Hui-Jun.(2015).Co-expression of xerophyte Zygophyllum xanthoxylum ZxNHX and ZxVP1-1 confers enhanced salinity tolerance in chimeric sugar beet (Beta vulgaris L.).FRONTIERS IN PLANT SCIENCE,6.
MLA	Wu, Guo-Qiang,et al."Co-expression of xerophyte Zygophyllum xanthoxylum ZxNHX and ZxVP1-1 confers enhanced salinity tolerance in chimeric sugar beet (Beta vulgaris L.)".FRONTIERS IN PLANT SCIENCE 6(2015).