Ultrasound-Assisted Aqueous Two-Phase System for Extraction and Enrichment of Zanthoxylum armatum Lignans

doi:10.3390/molecules200815273

	Ultrasound-Assisted Aqueous Two-Phase System for Extraction and Enrichment of Zanthoxylum armatum Lignans
	Guo, Tao 1,2; Su, Dan 1; Huang, Yan 1; Wang, Ya 1; Li, Yong-Hui 2
刊名	MOLECULES
	2015-08
卷号	20 期号:8 页码:15273-15286
关键词	Zanthoxylum armatum lignans aqueous two-phase system ultrasonic-assisted extraction artificial neural network response surface methodology
ISSN号	1420-3049
DOI	10.3390/molecules200815273
英文摘要	In the study, an aqueous two phase system (ATPS) coupled with ultrasound was employed to extract lignans from Zanthoxylum armatum. Three standard lignans, namely (-)-fargesin, sesamin and L-asarinin, were used as marker compounds, and extraction was optimized and projected by response surface methodology (RSM) and artificial neural network (ANN). The optimal condition for ATPS with 20% n-propanol and 24% (NH4)(2)SO4 coupled with ultrasonic-assisted extraction including a solvent to solid ratio of 15:1, a temperature of 40 degrees C, and a treatment time of 55 min was obtained. Under the condition, the yield of (-)-fargesin increased 15.12%, and the purities of (-)-fargesin, sesamin and L-asarinin reached 2.222%, 1.066%, and 1.583%, with an increase of 44.38%, 25.70%, and 26.34% compared to those extracted with 95% ethanol, respectively. Coefficient of the determined (0.9855) and mean squared error (0.0018) of ANN model suggested good fitness and generalization of the ANN. Taken together, the results showed that ultrasonic-assisted ATPS can be a suitable method for extraction and enrichment of lignans from Z. armatum.
资助项目	National Natural Science Foundation of China[81360476]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	MDPI
WOS记录号	WOS:000361375400107
状态	已发表
内容类型	期刊论文
源URL	[http://119.78.100.223/handle/2XXMBERH/34007]
专题	生命科学与工程学院
通讯作者	Li, Yong-Hui
作者单位	1.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Peoples R China 2.Hainan Med Univ, Hainan Prov Key Lab R&D Trop Plants, Haikou 571101, Peoples R China
推荐引用方式 GB/T 7714	Guo, Tao,Su, Dan,Huang, Yan,et al. Ultrasound-Assisted Aqueous Two-Phase System for Extraction and Enrichment of Zanthoxylum armatum Lignans[J]. MOLECULES,2015,20(8):15273-15286.
APA	Guo, Tao,Su, Dan,Huang, Yan,Wang, Ya,&Li, Yong-Hui.(2015).Ultrasound-Assisted Aqueous Two-Phase System for Extraction and Enrichment of Zanthoxylum armatum Lignans.MOLECULES,20(8),15273-15286.
MLA	Guo, Tao,et al."Ultrasound-Assisted Aqueous Two-Phase System for Extraction and Enrichment of Zanthoxylum armatum Lignans".MOLECULES 20.8(2015):15273-15286.