A Peroxidase-linked Spectrophotometric Assay for the Detection of Monoamine Oxidase Inhibitors

	A Peroxidase-linked Spectrophotometric Assay for the Detection of Monoamine Oxidase Inhibitors
	Zhi, Kangkang 1; Yang, Zhongduo 1; Sheng, Jie 1; Shu, Zongmei 1; Shi, Yin 2
刊名	IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH
	2016-12
卷号	15 期号:1 页码:131-139
关键词	Monoamine oxidase inhibitors Amine substrate Spectrophotometric Assay HPLC
ISSN号	1735-0328
英文摘要	To develop a new more accurate spectrophotometric method for detecting monoamine oxidase inhibitors from plant extracts, a series of amine substrates were selected and their ability to be oxidized by monoamine oxidase was evaluated by the HPLC method and a new substrate was used to develop a peroxidase-linked spectrophotometric assay. 4-(Trifluoromethyl) benzylamine (11) was proved to be an excellent substrate for peroxidase-linked spectrophotometric assay. Therefore, a new peroxidase-linked spectrophotometric assay was set up. The principle of the method is that the MAO converts 11 into aldehyde, ammonia and hydrogen peroxide. In the presence of peroxidase, the hydrogen peroxide will oxidize 4-aminoantipyrine into oxidised 4-aminoantipyrine which can condense with vanillic acid to give a red quinoneimine dye. The production of the quinoneimine dye was detected at 490 nm by a microplate reader. The. OD value between the blank group and blank negative control group in this new method is twice as much as that in Holt's method, which enables the procedure to be more accurate and avoids the produce of false positive results. The new method will be helpful for researchers to screening monoamine oxidase inhibitors from deep-color plant extracts.
资助项目	Elitist Program of Lanzhou University of Technology[J201303]
WOS研究方向	Pharmacology & Pharmacy
语种	英语
出版者	SHAHEED BEHESHTI UNIV, SCH PHARMACY
WOS记录号	WOS:000371506400010
状态	已发表
内容类型	期刊论文
源URL	[http://119.78.100.223/handle/2XXMBERH/33529]
专题	生命科学与工程学院
通讯作者	Yang, Zhongduo
作者单位	1.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Peoples R China 2.CAAS, Lanzhou Inst Husb & Pharmaceut Sci, Key Lab Vet Pharmaceut Dev, Key Lab New Anim Drug Project Gansu Prov,Minist A, Lanzhou 730050, Peoples R China
推荐引用方式 GB/T 7714	Zhi, Kangkang,Yang, Zhongduo,Sheng, Jie,et al. A Peroxidase-linked Spectrophotometric Assay for the Detection of Monoamine Oxidase Inhibitors[J]. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH,2016,15(1):131-139.
APA	Zhi, Kangkang,Yang, Zhongduo,Sheng, Jie,Shu, Zongmei,&Shi, Yin.(2016).A Peroxidase-linked Spectrophotometric Assay for the Detection of Monoamine Oxidase Inhibitors.IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH,15(1),131-139.
MLA	Zhi, Kangkang,et al."A Peroxidase-linked Spectrophotometric Assay for the Detection of Monoamine Oxidase Inhibitors".IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH 15.1(2016):131-139.