Polysaccharide from wheat bran induces cytokine expression via the toll-like receptor 4-mediated p38 MAPK signaling pathway and prevents cyclophosphamide-induced immunosuppression in mice

doi:10.1080/16546628.2017.1344523

	Polysaccharide from wheat bran induces cytokine expression via the toll-like receptor 4-mediated p38 MAPK signaling pathway and prevents cyclophosphamide-induced immunosuppression in mice
	Shen, Ting 3; Wang, Gongcheng 4; You, Long 3; Zhang, Liang 3; Ren, Haiwei 1; Hu, Weicheng 3; Qiang, Qian 3; Wang, Xinfeng 3; Jia, Lilian 3; Gu, Zhengzhong 2,3
刊名	FOOD & NUTRITION RESEARCH
	2017-07-09
卷号	61
关键词	Wheat bran polysaccharide immunomodulatory activity RAW 264.7 macrophages toll-like receptor 4
ISSN号	1654-6628
DOI	10.1080/16546628.2017.1344523
英文摘要	Wheat bran-derived polysaccharides have attracted particular attention due to their immunomodulatory effects. However, the molecular mechanisms underlying their functions are poorly understood. The current study was designed to examine the effect of wheat bran polysaccharide (WBP) on RAW 264.7 cells and the underlying signaling pathways, which have not been explored. In addition, we also investigated the immuno-enhancement effects of WBP on cyclophosphamide (CTX)-induced immunosuppression in mice. WBP significantly increased the concentrations of intracellular nitric oxide (NO) and cytokines such as prostaglandin E-2 (PGE2) and tumor necrosis factor-a (TNF-alpha) in RAW 264.7 cells. The result of RT-PCR analysis indicated that WBP also enhanced inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and TNF-a expression. Further analyses demonstrated that WBP rapidly activated phosphorylated p38 mitogen-activated protein kinase (MAPK) and the transcriptional activities of activator protein-1 (AP-1) and nuclear factor (NF)-kappa B via toll-like receptor 4 (TLR4). Furthermore, in vivo experiments revealed that WBP increased the spleen and thymus indices significantly, and markedly promoted the production of the serum cytokines IL-2 and IFN-v in CTX-induced immunosuppressed mice. Taken together, these results suggest that WBP can improve immunity by enhancing immune function, and could be explored as a potential immunomodulatory agent in functional food.
资助项目	Top-notch Academic Programs Project of Jiangsu Higher Education Institutions[PPZY2015A018]
WOS研究方向	Food Science & Technology ; Nutrition & Dietetics
语种	英语
出版者	SWEDISH NUTRITION FOUNDATION-SNF
WOS记录号	WOS:000406219200001
状态	已发表
内容类型	期刊论文
源URL	[http://119.78.100.223/handle/2XXMBERH/33192]
专题	生命科学与工程学院
通讯作者	Gu, Zhengzhong; Zhao, Xiangxiang
作者单位	1.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou, Gansu, Peoples R China 2.Huaiyin Inst Agr Sci Xuhuai Reg, Huaian, Peoples R China 3.Huaiyin Normal Univ, Jiangsu Key Lab Ecoagr Biotechnol Hongze Lake, Jiangsu Collaborat Innovat Ctr Reg Modern Agr & E, Huaian, Peoples R China 4.Nanjing Med Univ, Huaian Peoples Hosp 1, Dept Urol, Huaian, Peoples R China
推荐引用方式 GB/T 7714	Shen, Ting,Wang, Gongcheng,You, Long,et al. Polysaccharide from wheat bran induces cytokine expression via the toll-like receptor 4-mediated p38 MAPK signaling pathway and prevents cyclophosphamide-induced immunosuppression in mice[J]. FOOD & NUTRITION RESEARCH,2017,61.
APA	Shen, Ting.,Wang, Gongcheng.,You, Long.,Zhang, Liang.,Ren, Haiwei.,...&Zhao, Xiangxiang.(2017).Polysaccharide from wheat bran induces cytokine expression via the toll-like receptor 4-mediated p38 MAPK signaling pathway and prevents cyclophosphamide-induced immunosuppression in mice.FOOD & NUTRITION RESEARCH,61.
MLA	Shen, Ting,et al."Polysaccharide from wheat bran induces cytokine expression via the toll-like receptor 4-mediated p38 MAPK signaling pathway and prevents cyclophosphamide-induced immunosuppression in mice".FOOD & NUTRITION RESEARCH 61(2017).