Investigating immunosensor for determination of depression marker-Apo-A4 based on patterning AuNPs and N-Gr nanomaterials onto ITO-PET flexible electrodes with amplifying signal | |
Wang Yanping; Sun Bolu; Wei Hong; Li Yuanyuan; Hu Fangdi; Du Xing; Chen Junhui | |
刊名 | Analytica Chimica Acta |
2022-07-26 | |
卷号 | 1224期号:340217 |
关键词 | ITO-PET films Nitrogen-doped graphene (N-Gr) Flexible electrodes Human apolipoprotein A4 (Apo-A4) Electrochemical immunosensor |
英文摘要 | Developing wearable flexible biosensors with excellent electrical conductivity and outstanding flexibility simultaneously for disease diagnosis and health monitoring is a current hot topic in the field of sensor research. In this study, nitrogen-doped graphene (N-Gr) and gold nanoparticles (AuNPs) were successively deposited onto ITO-PET flexible conductive films by chemical deposition, and then a flexible electrochemical immunosensor with high sensitivity and selectivity was constructed for detecting depression markers by exploiting the high affinity between AuNPs and the sulfhydryl groups of depression marker (DM) antibody. Due to the composite of N-Gr, AuNPs and ITO-PET, the prepared flexible sensor can maintain a relatively stable electrical signal response regardless of the deformation such as spiral, rolling, and bending, without shedding or fracture of the N-Gr and AuNPs, and can also significantly amplify the electrochemical response signal of the immunosensor. Under optimized experimental conditions, the fabricated immunosensor showed good linearity over a wide range of 0.023–300.00 ng/mL with a low detection limit of 0.010 ng/mL (3σ, n = 5), when it was used for the determination of depression markers-human Apo-A4 in 100% whole serum samples. The flexibility of the constructed immunosensor and the stability and sensitivity of biomolecular analysis are expected to be further made into implantable depression marker in-situ monitoring probe with the help of micro-machining technology, which will develop a promising method for objective, efficient and accurate clinical diagnosis of depression. |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.lut.edu.cn/handle/2XXMBERH/159485] |
专题 | 生命科学与工程学院 |
通讯作者 | Hu Fangdi; Du Xing; Chen Junhui |
作者单位 | 1.School of Pharmacy, State Key Laboratory of Applied Organic Chemistry, Collaborative Innovation Center for Northwestern Chinese Medicine, Lanzhou University, Lanzhou, 730000, China 2.School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, 730050, China 3.School of Pharmacy, State Key Laboratory of Applied Organic Chemistry, Collaborative Innovation Center for Northwestern Chinese Medicine, Lanzhou University, Lanzhou, 730000, China 4.School of Pharmacy, State Key Laboratory of Applied Organic Chemistry, Collaborative Innovation Center for Northwestern Chinese Medicine, Lanzhou University, Lanzhou, 730000, China 5.School of Pharmacy, State Key Laboratory of Applied Organic Chemistry, Collaborative Innovation Center for Northwestern Chinese Medicine, Lanzhou University, Lanzhou, 730000, China 6.Gansu Agriculture Product Quality Safety Inspection Testing Center, Lanzhou, 730050, China 7.Department of Pediatrics, Gansu Province People’s Hospital, 204 Donggang West Road, Lanzhou, 730000, PR China |
推荐引用方式 GB/T 7714 | Wang Yanping,Sun Bolu,Wei Hong,et al. Investigating immunosensor for determination of depression marker-Apo-A4 based on patterning AuNPs and N-Gr nanomaterials onto ITO-PET flexible electrodes with amplifying signal[J]. Analytica Chimica Acta,2022,1224(340217). |
APA | Wang Yanping.,Sun Bolu.,Wei Hong.,Li Yuanyuan.,Hu Fangdi.,...&Chen Junhui.(2022).Investigating immunosensor for determination of depression marker-Apo-A4 based on patterning AuNPs and N-Gr nanomaterials onto ITO-PET flexible electrodes with amplifying signal.Analytica Chimica Acta,1224(340217). |
MLA | Wang Yanping,et al."Investigating immunosensor for determination of depression marker-Apo-A4 based on patterning AuNPs and N-Gr nanomaterials onto ITO-PET flexible electrodes with amplifying signal".Analytica Chimica Acta 1224.340217(2022). |
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