Energy metabolism as the target of 3-phenyllactic acid against Rhizopus oryzae

doi:10.1016/j.ijfoodmicro.2022.109606

	Energy metabolism as the target of 3-phenyllactic acid against Rhizopus oryzae
	Fan, Wenguang 1,2; Li, Baoyu 2; Du, Nana 2; Hui, Tian 2; Cao, Yingying 2; Li, Xin 2; Ren, Haiwei 2
刊名	INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
	2022-05-16
卷号	369
关键词	3-phenyllactic acid Rhizopus oryzae Anti-fungal mechanism Energy metabolism
ISSN号	0168-1605
DOI	10.1016/j.ijfoodmicro.2022.109606
英文摘要	3-phenyllactic acid (PLA) has broad anti-fungal activity, however, target sites of PLA on fungal cells and its antifungal mechanism of action have been poorly studied. In this study, we explored the inhibition mechanism of Rhizopus oryzae (R. oryzae) on rotten lily bulbs by PLA. The minimum inhibitory concentration value of PLA against R. oryzae was 8 mg/mL. We observed the ultrastructure of R. oryzae by scanning electron microscopy and transmission electron microscopy which indicated that PLA did not damage the cell membrane, but destroyed the mitochondria and other organelles. Tandem mass tag proteomes showed that PLA significantly down-regulated (P < 0.05) the expression of hexokinase (HK), phosphofructokinase (PFK), a-ketoglutarate dehydrogenase (aKGDH), adenylate kinase (ADK1), Cytochrome C oxidase and NADH dehydrogenase, and up-regulated (P < 0.05) the expression of mitochondrial ADP/ATP carrier proteins (AAC) and subunit IV (CCIO IV) in glycolysis, tricarboxylic acid cycle or oxidative phosphorylation metabolism. Following these findings, down-regulated HK and a-KGDH activity of aforementioned pathways was shown by enzyme activity assay, and regulated gene expression of ADK1, AAC, CCIO IV and NADH dehydrogenase was further confirmed by real-time quantitative PCR. Central carbon metabolomics showed that citric acid, cis-Aconitic acid, isocitric acid, alpha-Ketoglutaric acid, succinate, fumarate and malic acid of the tricarboxylic acid cycle metabolites were significantly downregulated (P < 0.05), and ATP production by oxidative phosphorylation was also significantly reduced (P = 0.02), resulting in insufficient energy production. Thus, ROS levels increased by 141% of the control values and cytochrome C was released, resulting in gradual cell apoptosis. All data indicated that energy metabolism was the target of PLA against R. oryzae. This was the first study to show that energy metabolism could be the target of PLA against R. oryzae, which could provide a theoretical basis to study the mechanism of fungal inhibition.
WOS研究方向	Food Science & Technology ; Microbiology
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000773003600003
内容类型	期刊论文
源URL	[http://ir.lut.edu.cn/handle/2XXMBERH/158647]
专题	生命科学与工程学院
作者单位	1.Lanzhou Univ Technol, Sch Life Sci & Engn, 287 Langongping Rd, Qilihe Dist, Lanzhou, Gansu, Peoples R China 2.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou, Gansu, Peoples R China;
推荐引用方式 GB/T 7714	Fan, Wenguang,Li, Baoyu,Du, Nana,et al. Energy metabolism as the target of 3-phenyllactic acid against Rhizopus oryzae[J]. INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY,2022,369.
APA	Fan, Wenguang.,Li, Baoyu.,Du, Nana.,Hui, Tian.,Cao, Yingying.,...&Ren, Haiwei.(2022).Energy metabolism as the target of 3-phenyllactic acid against Rhizopus oryzae.INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY,369.
MLA	Fan, Wenguang,et al."Energy metabolism as the target of 3-phenyllactic acid against Rhizopus oryzae".INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY 369(2022).