Expression of Aspergillus niger glucose oxidase in Pichia pastoris and its antimicrobial activity against Agrobacterium and Escherichia coli

doi:10.7717/peerj.9010

	Expression of Aspergillus niger glucose oxidase in Pichia pastoris and its antimicrobial activity against Agrobacterium and Escherichia coli
	Wang, Yonggang 1,2; Wang, Jiangqin 1,2; Leng, Feifan 1,2; Ma, Jianzhong 1,2; Bagadi, Alnoor 2
刊名	PEERJ
	2020-08-04
卷号	8
关键词	Aspergillus niger Pichia pastoris Glucose oxidase Transgenic Antimicrobial activity
ISSN号	2167-8359
DOI	10.7717/peerj.9010
英文摘要	The gene encoding glucose oxidase from Aspergillus niger ZM-8 was cloned and transferred to Pichia pastoris GS115, a transgenic strain P. pastoris GS115-His-GOD constructed. The growth curve of P. pastoris GS115-His-GOD was consistent with that of Pichia pastoris GS115-pPIC9K under non-induced culture conditions. Under methanol induction conditions, the growth of the GOD-transgenic strain was significantly lowered than P. pastoris GS115-pPIC9K with the induced-culture time increase, and the optical densities of GOD-transgenic strain reached one-third of that of the P. pastoris GS115-pPIC9K at 51 h. The activity of glucose oxidase in the cell-free supernatant, the supernatant of cell lysate, and the precipitation of cell lysate was 14.3 U/mL, 18.2 U/mL and 0.48 U/mL, respectively. The specific activity of glucose oxidase was 8.3 U/mg, 6.52 U/mg and 0.73 U/mg, respectively. The concentration of hydrogen peroxide formed by glucose oxidase from supernatant of the fermentation medium, the supernatant of the cell lysate, and the precipitation of cell lysate catalyzing 0.2 M glucose was 14.3 mu g/mL, 18.2 mu g/mL, 0.48 mu g/mL, respectively. The combination of different concentrations of glucose oxidase and glucose could significantly inhibit the growth of Agrobacterium and Escherichia coli in logarithmic phase. The filter article containing supernatant of the fermentation medium, supernatant of the cell lysate, and precipitation of cell lysate had no inhibitory effect on Agrobacterium and E. coli. The minimum inhibitory concentration of hydrogen peroxide on the plate culture of Agrobacterium and E. coli was 5.6 x 10(3) mu g/mL and 6.0 x 10(3) mu g/mL, respectively.
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	PEERJ INC
WOS记录号	WOS:000555317300001
内容类型	期刊论文
源URL	[http://ir.lut.edu.cn/handle/2XXMBERH/155124]
专题	生命科学与工程学院
作者单位	1.Lanzhou Univ Technol, Key Lab Drug Screening & Deep Proc Tradit Chinese, Lanzhou, Gansu, Peoples R China 2.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou, Gansu, Peoples R China;
推荐引用方式 GB/T 7714	Wang, Yonggang,Wang, Jiangqin,Leng, Feifan,et al. Expression of Aspergillus niger glucose oxidase in Pichia pastoris and its antimicrobial activity against Agrobacterium and Escherichia coli[J]. PEERJ,2020,8.
APA	Wang, Yonggang,Wang, Jiangqin,Leng, Feifan,Ma, Jianzhong,&Bagadi, Alnoor.(2020).Expression of Aspergillus niger glucose oxidase in Pichia pastoris and its antimicrobial activity against Agrobacterium and Escherichia coli.PEERJ,8.
MLA	Wang, Yonggang,et al."Expression of Aspergillus niger glucose oxidase in Pichia pastoris and its antimicrobial activity against Agrobacterium and Escherichia coli".PEERJ 8(2020).