A sensitive immunosensor based on graphene-PAMAM composites for rapid detection of the CP4-EPSPS protein in genetically modified crops

doi:10.1016/j.foodchem.2021.129901

	A sensitive immunosensor based on graphene-PAMAM composites for rapid detection of the CP4-EPSPS protein in genetically modified crops
	Zeng, Haijuan 2,4; Yang, Qianwen 1,2; Liu, Hua 2,4; Wu, Guogan 2,4; Jiang, Wei 2,4; Liu, Xiaofeng 1; Wang, Jinbin 2,4; Tang, Xueming 2,3,4
刊名	FOOD CHEMISTRY
	2021-11-01
卷号	361
关键词	Immunosensor Gold nanoparticle-antibody Graphene and PAMAM composites Genetically modified crops
ISSN号	0308-8146
DOI	10.1016/j.foodchem.2021.129901
英文摘要	A simple electrochemical immunosensor based on nitrogen-doped graphene and polyamide-amine (GN-PAM) composites was proposed for the detection of the CP4-EPSPS protein in genetically modified (GM) crops. In this immunosensor, the amplification of the detection signal was realized through antibodies labeled with gold nanoparticles (AuNPs). The electrochemical responses of the immunosensor were linear (R-2 = 0.9935 and 0.9912) when the GM soybean RRS and maize NK603 content ranged from 0.025% to 1.0% and 0.05% to 1.5%, respectively. The limits of detection for the GM soybean RRS and maize NK603 were as low as 0.01% and 0.03%, respectively. The immunosensor also exhibited high specificity, and satisfactory stability, reproducibility, and accuracy. Our findings indicated that the constructed immunosensor provides a new approach for the sensitive detection of the CP4-EPSPS protein. Notably, the sensor may be applied to other proteins or pathogenic bacteria by simply changing the antibodies, and may also be used for multi-component analysis.
WOS研究方向	Chemistry ; Food Science & Technology ; Nutrition & Dietetics
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000664737400003
内容类型	期刊论文
源URL	[http://ir.lut.edu.cn/handle/2XXMBERH/148999]
专题	生命科学与工程学院
作者单位	1.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Peoples R China; 2.Shanghai Acad Agr Sci, Biotechnol Res Inst, Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China; 3.Shanghai Jiao Tong Univ, Sch Agr & Biol, Shanghai 200240, Peoples R China 4.Minist Agr & Rural Affairs, Crops Ecol Environm Secur Inspect & Supervis Ctr, Shanghai 201106, Peoples R China;
推荐引用方式 GB/T 7714	Zeng, Haijuan,Yang, Qianwen,Liu, Hua,et al. A sensitive immunosensor based on graphene-PAMAM composites for rapid detection of the CP4-EPSPS protein in genetically modified crops[J]. FOOD CHEMISTRY,2021,361.
APA	Zeng, Haijuan.,Yang, Qianwen.,Liu, Hua.,Wu, Guogan.,Jiang, Wei.,...&Tang, Xueming.(2021).A sensitive immunosensor based on graphene-PAMAM composites for rapid detection of the CP4-EPSPS protein in genetically modified crops.FOOD CHEMISTRY,361.
MLA	Zeng, Haijuan,et al."A sensitive immunosensor based on graphene-PAMAM composites for rapid detection of the CP4-EPSPS protein in genetically modified crops".FOOD CHEMISTRY 361(2021).