Optimization and mechanism exploration for Escherichia coli transformed with plasmid pUC19 by the combination with ultrasound treatment and chemical method

doi:10.1016/j.ultsonch.2021.105552

	Optimization and mechanism exploration for Escherichia coli transformed with plasmid pUC19 by the combination with ultrasound treatment and chemical method
	Wang, Yonggang 1; Sun, Shangchen 2; Yu, Linmiao 1; Hu, Shu 1; Fan, Wenguang 1; Leng, Feifan 1; Ma, Jianzhong 1
刊名	Ultrasonics Sonochemistry
	2021-06-01
卷号	74
关键词	Clone cells Cytology Efficiency Escherichia coli Food processing Ultrasonic applications Chemical method Genetic transformation Host cells Molecular cloning Optimisations Plasmid puc19 Transformation efficiency Transformation systems Treatment methods Ultrasound treatments
ISSN号	1350-4177
DOI	10.1016/j.ultsonch.2021.105552
英文摘要	As a basic technique of molecular cloning, bio-transformation has been successfully used in the fields of biomedicine and food processing. In this study, we established a transformation system of exogenous DNA into E. coli cells mediated by ultrasound. Under the optimal conditions (i.e. 35 °C, 40 W, 25 s, OD600 = 0.4–0.6) optimized by RSM, the transformation efficiency reached at 1.006 × 107 CFU/μg DNA. The results of membrane permeability, macromolecular substance and cell structure analysis before and after ultrasound treatment showed that the damage of host cells induced by lower (40 W) ultrasound and shorter ultrasound time (25 s) was reversible, and the transformation efficiency and cell survival rate were not significantly affected under this condition. In brief, proper changes in cell membrane and cell wall were the basic conditions for host cells to uptake exogenous DNA, while, whether exogenous DNA could be replicated and expressed in cells depends on the viability of host cells. © 2021 The Author(s)
WOS研究方向	Acoustics ; Chemistry
语种	英语
出版者	Elsevier B.V.
WOS记录号	WOS:000663409800004
内容类型	期刊论文
源URL	[http://ir.lut.edu.cn/handle/2XXMBERH/148418]
专题	生命科学与工程学院
作者单位	1.School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou; 730050, China; 2.School of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou; 730050, China
推荐引用方式 GB/T 7714	Wang, Yonggang,Sun, Shangchen,Yu, Linmiao,et al. Optimization and mechanism exploration for Escherichia coli transformed with plasmid pUC19 by the combination with ultrasound treatment and chemical method[J]. Ultrasonics Sonochemistry,2021,74.
APA	Wang, Yonggang.,Sun, Shangchen.,Yu, Linmiao.,Hu, Shu.,Fan, Wenguang.,...&Ma, Jianzhong.(2021).Optimization and mechanism exploration for Escherichia coli transformed with plasmid pUC19 by the combination with ultrasound treatment and chemical method.Ultrasonics Sonochemistry,74.
MLA	Wang, Yonggang,et al."Optimization and mechanism exploration for Escherichia coli transformed with plasmid pUC19 by the combination with ultrasound treatment and chemical method".Ultrasonics Sonochemistry 74(2021).