Changes in hydrolysis specificities of lipase from Rhizomucor miehei to polyunsaturated fatty acyl ethyl esters in different aggregation states

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	Changes in hydrolysis specificities of lipase from Rhizomucor miehei to polyunsaturated fatty acyl ethyl esters in different aggregation states
	Kosugi, Y. ; Chang, Q. L. ; Kanazawa, K. ; Nakanishi, H.
刊名	Journal of the American Oil Chemists Society
	1997
期号	11 页码:1395-1399
关键词	aggregation fatty acid immobilized lipase interfacial activation lipase polyunsaturated fatty acid specificity organic media interfacial activation acids oil immobilization
其他题名	J. Am. Oil Chem. Soc.
中文摘要	Hydrolysis specificities of lipase from Rhizomucor miehei were compared for various fatty acyl ethyl esters. Activity yields of immobilized lipases, measured with 1 mM substrate, were more than 100%. Differences in hydrolysis rate and affinity for the substrates between lipase preparations were also typically higher during hydrolysis of substrates at 100 mM than at 1 mM, indicating better mass transfer effects for 1-mM substrates. The native lipase showed higher affinity for polyunsaturated fatty acid substrates at 1 mM than at 100 mM. Hydrolysis rates for 1-mM substrates were observed with immobilized lipases, fixed on anion exchange resin with glutaraldehyde and on cation exchange carrier with carbodiimide, and suggested some modification of the basic amino acid related to the lid of R. miehei lipase. Activation with these bifunctional reagents was not observed for 100-mM substrates, indicating that interfacial activation always occurred because of aggregation of 100-mM substrates. These results show that lipase from R. miehei recognizes molecular aggregation of lipids, and that various changes occur in the hydrolysis specificities for fatty acids.
收录类别	SCI
原文出处	://WOS:A1997YG66100008
语种	英语
公开日期	2013-11-15
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/5872]
专题	过程工程研究所_研究所（批量导入）
推荐引用方式 GB/T 7714	Kosugi, Y.,Chang, Q. L.,Kanazawa, K.,et al. Changes in hydrolysis specificities of lipase from Rhizomucor miehei to polyunsaturated fatty acyl ethyl esters in different aggregation states[J]. Journal of the American Oil Chemists Society,1997(11):1395-1399.
APA	Kosugi, Y.,Chang, Q. L.,Kanazawa, K.,&Nakanishi, H..(1997).Changes in hydrolysis specificities of lipase from Rhizomucor miehei to polyunsaturated fatty acyl ethyl esters in different aggregation states.Journal of the American Oil Chemists Society(11),1395-1399.
MLA	Kosugi, Y.,et al."Changes in hydrolysis specificities of lipase from Rhizomucor miehei to polyunsaturated fatty acyl ethyl esters in different aggregation states".Journal of the American Oil Chemists Society .11(1997):1395-1399.