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	Affinity extraction of BSA by mixed reversed micellar system with unbound triazine dye
	Zhang, TX; Liu, HZ; Chen, JY
刊名	BIOCHEMICAL ENGINEERING JOURNAL
	1999-09-01
卷号	4期号:1页码:17-21
关键词	affinity extraction reversed micelles Cibacron Blue 3GA bovine serum albumin
ISSN号	1369-703X
其他题名	Biochem. Eng. J.
中文摘要	The affinity extraction of proteins with unbound triazine dye in the mixed reversed micellar system has been studied. Bovine serum albumin (BSA) and Cibacron Blue 3GA (CB) were chosen, respectively, as a model protein and an unbound ligand. The affinity CB in the aqueous phase was directly transferred to the mixed reversed micelles formed with cationic surfactant cetyltrimethylammonium bromide (CTAB) and tributyl phosphate (TBP). The transfer of BSA to the reversed micelles increases significantly by the biospecific interaction with the addition of a small amount of CB to the aqueous phase. For solutions with pH < pI, the selectivity of proteins can be expected with the presence of affinity CB since no BSA was found to be extracted into the reverse micelles without the addition of CB. The BSA transfer to the reversed micelles increases significantly by the addition of TBP to the organic phase. Most of BSA (similar to 82%) extracted into the reversed micelles can be back extracted into the aqueous phase by the increase of n-hexanol concentration in the organic phase while no CB would be transferred into the stripping aqueous solution at the same time. (C) 1999 Elsevier Science S.A. All rights reserved.
英文摘要	The affinity extraction of proteins with unbound triazine dye in the mixed reversed micellar system has been studied. Bovine serum albumin (BSA) and Cibacron Blue 3GA (CB) were chosen, respectively, as a model protein and an unbound ligand. The affinity CB in the aqueous phase was directly transferred to the mixed reversed micelles formed with cationic surfactant cetyltrimethylammonium bromide (CTAB) and tributyl phosphate (TBP). The transfer of BSA to the reversed micelles increases significantly by the biospecific interaction with the addition of a small amount of CB to the aqueous phase. For solutions with pH < pI, the selectivity of proteins can be expected with the presence of affinity CB since no BSA was found to be extracted into the reverse micelles without the addition of CB. The BSA transfer to the reversed micelles increases significantly by the addition of TBP to the organic phase. Most of BSA (similar to 82%) extracted into the reversed micelles can be back extracted into the aqueous phase by the increase of n-hexanol concentration in the organic phase while no CB would be transferred into the stripping aqueous solution at the same time. (C) 1999 Elsevier Science S.A. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]	Biotechnology & Applied Microbiology ; Engineering, Chemical
研究领域[WOS]	Biotechnology & Applied Microbiology ; Engineering
关键词[WOS]	PROTEIN EXTRACTION ; COSURFACTANT ; PURIFICATION ; SEPARATION ; ENZYMES
收录类别	SCI
原文出处	://WOS:000082915200003
语种	英语
WOS记录号	WOS:000082915200003
公开日期	2013-11-15
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/5853]
专题	过程工程研究所_研究所（批量导入）
作者单位	Chinese Acad Sci, Young Scientist Lab Separat Sci & Engn, Inst Chem Met, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Zhang, TX,Liu, HZ,Chen, JY. Affinity extraction of BSA by mixed reversed micellar system with unbound triazine dye[J]. BIOCHEMICAL ENGINEERING JOURNAL,1999,4(1):17-21.
APA	Zhang, TX,Liu, HZ,&Chen, JY.(1999).Affinity extraction of BSA by mixed reversed micellar system with unbound triazine dye.BIOCHEMICAL ENGINEERING JOURNAL,4(1),17-21.
MLA	Zhang, TX,et al."Affinity extraction of BSA by mixed reversed micellar system with unbound triazine dye".BIOCHEMICAL ENGINEERING JOURNAL 4.1(1999):17-21.

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