Separation and purification of hydrolyzable tannin from Geranium wilfordii Maxim by reversed-phase and normal-phase highspeed counter-current chromatography

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	Separation and purification of hydrolyzable tannin from Geranium wilfordii Maxim by reversed-phase and normal-phase highspeed counter-current chromatography
	Liu, Dan 2; Su, Zhiguo 1; Wang, Changhai 2; Gu, Ming 1; Xing, Siliang 3
刊名	JOURNAL OF SEPARATION SCIENCE
	2010-08-01
卷号	33 期号:15 页码:2266-2271
关键词	Corilagin Gallic acid Geraniin Normal-phase high-speed counter-current chromatography Reversed-phase high-speed counter-current chromatography
ISSN号	1615-9306
通讯作者	Gu, M
英文摘要	Three hydrolyzable tannins, geraniin, corilagin and gallic acid, main active components of Geranium wilfordii Maxim, have been separated and purified in one-step by both reversed-phase and normal-phase high-speed counter-current chromatography. Gallic acid, corilagin and geraniin were purified from 70% aqueous acetone extract of G. wilfordii Maxim with solvent system n-hexane-ethyl acetate-methanol-acetic acid-water (1:10:0.2:0.2:20) by reversed-phase high-speed counter-current chromatography at purities of 94.2, 91.0 and 91.3%, at yields of 89.3, 82.9 and 91.7%, respectively. Gallic acid, corilagin and geraniin were purified with solvent system n-hexane-ethyl acetate-methanol-acetic acid-water (0.2:10:2:1:5) by normal-phase high-speed counter-current chromatography at purities of 85.9, 92.2 and 87.6%, at yields of 87.4, 94.6 and 94.3%, respectively. It was successful for both reversed-phase and normal-phase high-speed counter-current chromatography to separate high-polarity of low-molecular-weight substances.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Analytical
研究领域[WOS]	Chemistry
关键词[WOS]	PHYLLANTHUS-URINARIA ; PARTITION CHROMATOGRAPHY ; PREPARATIVE ISOLATION ; GALLIC ACID ; BUNGE
收录类别	SCI
语种	英语
WOS记录号	WOS:000281055900006
公开日期	2013-11-15
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/6110]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Dalian Univ Technol, Sch Environm & Biol Sci & Technol, Dalian, Peoples R China 3.Beijing Kangmingyousheng Biochem Technol Co Ltd, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Liu, Dan,Su, Zhiguo,Wang, Changhai,et al. Separation and purification of hydrolyzable tannin from Geranium wilfordii Maxim by reversed-phase and normal-phase highspeed counter-current chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2010,33(15):2266-2271.
APA	Liu, Dan,Su, Zhiguo,Wang, Changhai,Gu, Ming,&Xing, Siliang.(2010).Separation and purification of hydrolyzable tannin from Geranium wilfordii Maxim by reversed-phase and normal-phase highspeed counter-current chromatography.JOURNAL OF SEPARATION SCIENCE,33(15),2266-2271.
MLA	Liu, Dan,et al."Separation and purification of hydrolyzable tannin from Geranium wilfordii Maxim by reversed-phase and normal-phase highspeed counter-current chromatography".JOURNAL OF SEPARATION SCIENCE 33.15(2010):2266-2271.