Molecular mechanisms of heavy metals resistance of Stenotrophomonas rhizophila JC1 by whole genome sequencing

doi:10.1007/s00203-021-02271-0

	Molecular mechanisms of heavy metals resistance of Stenotrophomonas rhizophila JC1 by whole genome sequencing
	Sun, Shang-Chen 1; Chen, Ji-Xiang 1; Wang, Yong-Gang 2; Leng, Fei-Fan 2; Zhao, Jian 2; Chen, Kai 2; Zhang, Qing-Chun 3
刊名	ARCHIVES OF MICROBIOLOGY
	2021
卷号	203 期号:5 页码:2699-2709
关键词	Stenotrophomonas rhizophila JC1 Heavy metals Genome Adsorption Detoxification
ISSN号	0302-8933
DOI	10.1007/s00203-021-02271-0
英文摘要	In this study, a higher metal ions-resistant bacterium, Stenotrophomonas rhizophila JC1 was isolated from contaminated soil in Jinchang city, Gansu Province, China. The Pb2+ (120 mg/L) and Cu2+ (80 mg/L) removal rate of the strain reached at 76.9% and 83.4%, respectively. The genome comprises 4268161 bp in a circular chromosome with 67.52% G + C content and encodes 3719 proteins. The genome function analysis showed czc operon, mer operon, cop operon, arsenic detoxification system in strain JC1 were contributed to the removal of heavy metals. Three efflux systems (i.e., RND, CDF, and P-ATPase) on strain JC1 genome could trigger the removal of divalent cations from cells. cAMP pathway and ABC transporter pathway might be involved in the transport and metabolism of heavy metals. The homology analysis exhibited multi-gene families such as ABC transporters, heavy metal-associated domain, copper resistance protein, carbohydrate-binding domain were distributed across 410 orthologous groups. In addition, heavy metal-responsive transcription regulator, thioredoxin, heavy metal transport/detoxification protein, divalent-cation resistance protein CutA, arsenate reductase also played important roles in the heavy metals adsorption and detoxification process. The complete genome data provides insight into the exploration of the interaction mechanism between microorganisms and heavy metals.
WOS研究方向	Microbiology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000628729400003
内容类型	期刊论文
源URL	[http://ir.lut.edu.cn/handle/2XXMBERH/148322]
专题	生命科学与工程学院石油化工学院
作者单位	1.Lanzhou Univ Technol, Sch Petrochem Engn, Langongping Rd 287, Lanzhou 730050, Peoples R China; 2.Lanzhou Univ Technol, Sch Life Sci & Engn, Langongping Rd 287, Lanzhou 730050, Peoples R China; 3.Agr Technol Extens Ctr Kang Cty, Longnan 746500, Kang County, Peoples R China
推荐引用方式 GB/T 7714	Sun, Shang-Chen,Chen, Ji-Xiang,Wang, Yong-Gang,et al. Molecular mechanisms of heavy metals resistance of Stenotrophomonas rhizophila JC1 by whole genome sequencing[J]. ARCHIVES OF MICROBIOLOGY,2021,203(5):2699-2709.
APA	Sun, Shang-Chen.,Chen, Ji-Xiang.,Wang, Yong-Gang.,Leng, Fei-Fan.,Zhao, Jian.,...&Zhang, Qing-Chun.(2021).Molecular mechanisms of heavy metals resistance of Stenotrophomonas rhizophila JC1 by whole genome sequencing.ARCHIVES OF MICROBIOLOGY,203(5),2699-2709.
MLA	Sun, Shang-Chen,et al."Molecular mechanisms of heavy metals resistance of Stenotrophomonas rhizophila JC1 by whole genome sequencing".ARCHIVES OF MICROBIOLOGY 203.5(2021):2699-2709.