Chip-Capillary Hybrid Device for Automated Transfer of Sample Preseparated by Capillary Isoelectric Focusing to Parallel Capillary Gel Electrophoresis for Two-Dimensional Protein Separation

doi:10.1021/ac3017168

CORC > 兰州理工大学 > 兰州理工大学 > 石油化工学院

	Chip-Capillary Hybrid Device for Automated Transfer of Sample Preseparated by Capillary Isoelectric Focusing to Parallel Capillary Gel Electrophoresis for Two-Dimensional Protein Separation
	Lu, Joann J.3; Wang, Shili 3; Li, Guanbin 1; Wang, Wei 3; Pu, Qiaosheng 2; Liu, Shaorong 3
刊名	ANALYTICAL CHEMISTRY
	2012-08-21
卷号	84 期号:16 页码:7001-7007
关键词	Automation Capillarity Capillary electrophoresis Proteins Separation Sulfur compounds
ISSN号	0003-2700
DOI	10.1021/ac3017168
英文摘要	In this article, we introduce a chip-capillary hybrid device to integrate capillary isoelectric focusing (CIEF) with parallel capillary sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS-PAGE) or capillary gel electrophoresis (CGE) toward automating two-dimensional (2D) protein separations. The hybrid device consists of three chips that are butted together. The middle chip can be moved between two positions to reroute the fluidic paths, which enables the performance of CLEF and injection of proteins partially resolved by CLEF to CGE capillaries for parallel CGE separations in a continuous and automated fashion. Capillaries are attached to the other two chips to facilitate CIEF and CGE separations and to extend the effective lengths of CGE columns. Specifically, we illustrate the working principle of the hybrid device, develop protocols for producing and preparing the hybrid device, and demonstrate the feasibility of using this hybrid device for automated injection of CLEF-separated sample to parallel CGE for 2D protein separations. Potentials and problems associated with the hybrid device are also discussed.
WOS研究方向	Chemistry
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000307695900014
内容类型	期刊论文
源URL	[http://119.78.100.223/handle/2XXMBERH/34863]
专题	石油化工学院
作者单位	1.Lanzhou Univ Technol, Sch Petrochem Engn, Lanzhou 730050, Gansu, Peoples R China 2.Lanzhou Univ, Dept Chem, Lanzhou 730000, Gansu, Peoples R China; 3.Univ Oklahoma, Dept Chem & Biochem, Norman, OK 73019 USA;
推荐引用方式 GB/T 7714	Lu, Joann J.,Wang, Shili,Li, Guanbin,et al. Chip-Capillary Hybrid Device for Automated Transfer of Sample Preseparated by Capillary Isoelectric Focusing to Parallel Capillary Gel Electrophoresis for Two-Dimensional Protein Separation[J]. ANALYTICAL CHEMISTRY,2012,84(16):7001-7007.
APA	Lu, Joann J.,Wang, Shili,Li, Guanbin,Wang, Wei,Pu, Qiaosheng,&Liu, Shaorong.(2012).Chip-Capillary Hybrid Device for Automated Transfer of Sample Preseparated by Capillary Isoelectric Focusing to Parallel Capillary Gel Electrophoresis for Two-Dimensional Protein Separation.ANALYTICAL CHEMISTRY,84(16),7001-7007.
MLA	Lu, Joann J.,et al."Chip-Capillary Hybrid Device for Automated Transfer of Sample Preseparated by Capillary Isoelectric Focusing to Parallel Capillary Gel Electrophoresis for Two-Dimensional Protein Separation".ANALYTICAL CHEMISTRY 84.16(2012):7001-7007.