A novel loop-mediated isothermal amplification method for detection of the carrot materials in foods

doi:10.1007/s00217-015-2459-1

CORC > 兰州理工大学 > 兰州理工大学 > 石油化工学院

	A novel loop-mediated isothermal amplification method for detection of the carrot materials in foods
	Sun, Min 1,2; Gao, Hongwei 2; Xiao, Xizhi 2; Chen, Jixiang 3; Liu, Caixia 2; Feng, Liping 2
刊名	EUROPEAN FOOD RESEARCH AND TECHNOLOGY
	2015-08
卷号	241 期号:2 页码:295-302
关键词	Carrot Allergen Detection Loop-mediated isothermal amplification
ISSN号	1438-2377
DOI	10.1007/s00217-015-2459-1
英文摘要	Carrot is an important elicitor of food allergy. In this study, we developed a novel molecular detection assay based on loop-mediated isothermal amplification technology for the detection of carrot in food materials. The assay had sensitivity up to 4 pg of the purified carrot DNA and had no cross-reactions with other species, such as fennel, parsley, anise, caraway, cumin, walnut, pecan, peanut, cashew, almond, pistachio, sunflower seed, sesame seed, chestnut, soy, barley, wheat, oat, rye, rice, cabbage, green pepper, onion, celery, cucumber, beef, mutton, pork, chicken and shrimp. The validation study demonstrated high reproducibility and specificity. This assay was proved to be a potential tool for the detection and label management of carrot allergens in foods.
资助项目	General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China[2011IK011] ; General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China[2009IK254]
WOS研究方向	Food Science & Technology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000358055100013
状态	已发表
内容类型	期刊论文
源URL	[http://119.78.100.223/handle/2XXMBERH/34009]
专题	石油化工学院
通讯作者	Sun, Min
作者单位	1.Ocean Univ China, Coll Marine Life Sci, Qingdao, Peoples R China 2.Shandong Entry Exit Inspect & Quarantine Bur, Ctr Tech, Qingdao, Peoples R China 3.Lanzhou Univ Technol, Sch Petrochem Engn, Lanzhou, Peoples R China
推荐引用方式 GB/T 7714	Sun, Min,Gao, Hongwei,Xiao, Xizhi,et al. A novel loop-mediated isothermal amplification method for detection of the carrot materials in foods[J]. EUROPEAN FOOD RESEARCH AND TECHNOLOGY,2015,241(2):295-302.
APA	Sun, Min,Gao, Hongwei,Xiao, Xizhi,Chen, Jixiang,Liu, Caixia,&Feng, Liping.(2015).A novel loop-mediated isothermal amplification method for detection of the carrot materials in foods.EUROPEAN FOOD RESEARCH AND TECHNOLOGY,241(2),295-302.
MLA	Sun, Min,et al."A novel loop-mediated isothermal amplification method for detection of the carrot materials in foods".EUROPEAN FOOD RESEARCH AND TECHNOLOGY 241.2(2015):295-302.