Liquid Chromatography-Tandem Mass Spectrometry Screening Method for the Detection of Radical-Scavenging Natural Antioxidants from the Whole Scutellariae (Radix, Stem and Leaf)

doi:10.1093/chromsci/bmu176

CORC > 兰州理工大学 > 兰州理工大学 > 石油化工学院

	Liquid Chromatography-Tandem Mass Spectrometry Screening Method for the Detection of Radical-Scavenging Natural Antioxidants from the Whole Scutellariae (Radix, Stem and Leaf)
	Shi, Gao-Feng; Yao, Rui-Xing; Wang, Guo-Ying; Wang, Zhen-Ju; Chen, Fu-Wen
刊名	JOURNAL OF CHROMATOGRAPHIC SCIENCE
	2015-08
卷号	53 期号:7 页码:1140-1146
ISSN号	0021-9665
DOI	10.1093/chromsci/bmu176
英文摘要	A novel free radical reaction combined with high-performance LC-photodiode array-ESI-MS/MS screening method was developed for the detection and identification of natural antioxidants from whole Scutellariae. Six compounds and whole Scutellariae extracts were found to possess a potential antioxidant capacity, and their free radical-scavenging activities were investigated in detail. The six compounds were identified as baicalin, baicalein, scutellarin, scutellarein, wogonoside and chrystin-7-glucoronide. The present study reveals that the radical-scavenging capacities of the whole Scutellariae extracts are as follows: hydroxyl radical > superoxide radical > peroxy radical. Wogonoside showed the strongest capability for scavenging hydroxyl radical. Baicalein not only showed the strongest capability for scavenging superoxide radical but also showed capability for lipid radical; Scutellarein (Peak 5) exhibited the highest reactivity in the lipid peroxidation processes. Based on these studies, the current paper accomplishes the evaluation of activities of some important anti-radical substances extracted from the radix, stem and leaf of the whole Scutellariae.
资助项目	National Natural Science Foundation of China[21407072] ; Natural Science Foundation of Gansu Province[145RJZA109] ; HongLiu Natural Science Foundation of Lanzhou University of Technology[Q201403]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	OXFORD UNIV PRESS INC
WOS记录号	WOS:000363983400013
状态	已发表
内容类型	期刊论文
源URL	[http://119.78.100.223/handle/2XXMBERH/33998]
专题	石油化工学院
通讯作者	Wang, Guo-Ying
作者单位	Lanzhou Univ Technol, Sch Petrochem Engn, Lanzhou 730050, Gansu, Peoples R China
推荐引用方式 GB/T 7714	Shi, Gao-Feng,Yao, Rui-Xing,Wang, Guo-Ying,et al. Liquid Chromatography-Tandem Mass Spectrometry Screening Method for the Detection of Radical-Scavenging Natural Antioxidants from the Whole Scutellariae (Radix, Stem and Leaf)[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE,2015,53(7):1140-1146.
APA	Shi, Gao-Feng,Yao, Rui-Xing,Wang, Guo-Ying,Wang, Zhen-Ju,&Chen, Fu-Wen.(2015).Liquid Chromatography-Tandem Mass Spectrometry Screening Method for the Detection of Radical-Scavenging Natural Antioxidants from the Whole Scutellariae (Radix, Stem and Leaf).JOURNAL OF CHROMATOGRAPHIC SCIENCE,53(7),1140-1146.
MLA	Shi, Gao-Feng,et al."Liquid Chromatography-Tandem Mass Spectrometry Screening Method for the Detection of Radical-Scavenging Natural Antioxidants from the Whole Scutellariae (Radix, Stem and Leaf)".JOURNAL OF CHROMATOGRAPHIC SCIENCE 53.7(2015):1140-1146.