Synthesis of mesoporous silica with different pore sizes for cellulase immobilization: pure physical adsorption

doi:10.1039/c7nj00441a

CORC > 兰州理工大学 > 兰州理工大学 > 石油化工学院

	Synthesis of mesoporous silica with different pore sizes for cellulase immobilization: pure physical adsorption
	Chen, Baiyi 1; Qiu, Jianhui 1; Mo, Haodao 1; Yu, Yanling 1,2; Ito, Kazushi 1; Sakai, Eiichi 1; Feng, Huixia 3
刊名	NEW JOURNAL OF CHEMISTRY
	2017-09-07
卷号	41 期号:17 页码:9338-9345
ISSN号	1144-0546
DOI	10.1039/c7nj00441a
英文摘要	To discuss the physical adsorption mechanism of the adsorption process of cellulase, a commercial enzyme cocktail sourced from Acremonium was immobilized in mesoporous silica with various pore sizes by pure physical adsorption in this study. Mesoporous silica materials with 17.6 nm and 3.8 nm pore sizes (hereafter referred to as MS-17.6 nm and MS-3.8 nm, respectively) were synthesized in the manner of a seeded-growth method. Other available mesoporous silica materials denoted as H-32 and diatomite were also used as sorbents. Then, the sorbents were characterized via small-angle X-ray scattering (SAXS), transmission electron microscopy (TEM), scanning electron microscopy (SEM), the Barrett-Emmett-Teller (BET) method and the Barrett-Joyner-Halenda (BJH) method to confirm their mesostructure. Furthermore, the adsorption abilities of different sorbents and enzymatic activities of immobilized cellulase were studied. The adsorption amounts exhibited a clear correlation with the pore size of the sorbents; i.e., the adsorption amount of MS-17.6 nm (410 mg g(-1)) with the pore size similar to the long axes of cellulase molecules was higher than that of MS-3.8 nm (315 mg g(-1)) with the pore size approximated to the short axes of cellulase (which was realized at 50 degrees C). Besides, the adsorption behavior of diatomite (with a pore size of about 200 nm) revealed a periodicity because the pore size was significantly larger than cellulase molecules. Moreover, the pore size was suggested to be a critical factor for the enzymatic activity of cellulase. When the average pore size of MS-3.8 nm just matched the short axes of cellulase molecules, immobilized cellulase preserved the active sites of cellulase intactly and showed the best activity (i.e. 63.3% of free cellulase activity at 50 degrees C). Consequently, the pore size of the sorbents had a significant influence on cellulase immobilization.
资助项目	International Collaboration Project - Shenzhen Government[GJHZ20150316160614843]
WOS研究方向	Chemistry
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000411762600077
状态	已发表
内容类型	期刊论文
源URL	[http://119.78.100.223/handle/2XXMBERH/33108]
专题	石油化工学院教务处（创新创业学院）
通讯作者	Qiu, Jianhui
作者单位	1.Akita Prefectural Univ, Fac Syst Engn, Dept Machine Intelligence & Syst Engn, Akita 0150055, Japan 2.Harbin Inst Technol, Sch Chem Engn & Technol, Harbin 150001, Heilongjiang, Peoples R China 3.Lanzhou Univ Technol, Coll Petrochem Technol, Lanzhou 730050, Gansu, Peoples R China
推荐引用方式 GB/T 7714	Chen, Baiyi,Qiu, Jianhui,Mo, Haodao,et al. Synthesis of mesoporous silica with different pore sizes for cellulase immobilization: pure physical adsorption[J]. NEW JOURNAL OF CHEMISTRY,2017,41(17):9338-9345.
APA	Chen, Baiyi.,Qiu, Jianhui.,Mo, Haodao.,Yu, Yanling.,Ito, Kazushi.,...&Feng, Huixia.(2017).Synthesis of mesoporous silica with different pore sizes for cellulase immobilization: pure physical adsorption.NEW JOURNAL OF CHEMISTRY,41(17),9338-9345.
MLA	Chen, Baiyi,et al."Synthesis of mesoporous silica with different pore sizes for cellulase immobilization: pure physical adsorption".NEW JOURNAL OF CHEMISTRY 41.17(2017):9338-9345.