RPA-Cas12a-FS: A frontline nucleic acid rapid detection system for food safety based on CRISPR-Cas12a combined with recombinase polymerase amplification

doi:10.1016/j.foodchem.2020.127608

	RPA-Cas12a-FS: A frontline nucleic acid rapid detection system for food safety based on CRISPR-Cas12a combined with recombinase polymerase amplification
	Liu, Hua 1,2; Wang, Jinbin 1,2; Zeng, Haijuan 1,2; Liu, Xiaofeng 3; Jiang, Wei 1,2; Wang, Yu 3; Ouyang, Wanbao 3; Tang, Xueming 1,2
刊名	Food Chemistry
	2021
卷号	334
关键词	Bacteria Nucleic acids Ancillary equipment Fluorescence intensities Food-borne pathogenic bacteria Genetically modified crops Highly sensitive detections Molecular identification Rapid detection systems Technical expertise
ISSN号	03088146
DOI	10.1016/j.foodchem.2020.127608
英文摘要	Food analysis to ensure food safety and quality are relevant to all countries. This study aimed to develop a detection technique by combining recombinase polymerase amplification with CRISPR-Cas12a for food safety (termed RPA-Cas12a-FS). Our data showed that this novel method could be detected via fluorescence intensity for the molecular identification of foodborne pathogenic bacteria, genetically modified crops, and meat adulteration. After optimization, the sensitivity and stability of RPA-Cas12a-FS was further enhanced. The RPA-Cas12a-FS system could specifically detect target gene levels as low as 10 copies in 45 min at 37 °C. The RPA-Cas12a-FS system was sensitive both using standard samples in the lab and using samples from the field, which indicated that this detection method was practical. In conclusion, a simple, rapid, and highly sensitive detection method based on CRISPR-Cas12a was developed for molecular identification in the food safety field without requiring technical expertise or ancillary equipment. © 2020 Elsevier Ltd
WOS研究方向	Chemistry ; Food Science & Technology ; Nutrition & Dietetics
语种	英语
出版者	Elsevier Ltd
WOS记录号	WOS:000571663500015
内容类型	期刊论文
源URL	[http://ir.lut.edu.cn/handle/2XXMBERH/147162]
专题	生命科学与工程学院能源与动力工程学院
作者单位	1.Institute of Biotechnology Research, Shanghai Academy of Agricultural Sciences; Key Laboratory of Agricultural Genetics and Breeding, 2901 Beidi Road, Shanghai; 201106, China; 2.Crops Ecological Environment Security Inspection and Supervision Center (Shanghai), Ministry of Agriculture and Rural Affairs, P.R.C., 2901 Beidi Road, Shanghai; 201106, China; 3.School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou; 730050, China
推荐引用方式 GB/T 7714	Liu, Hua,Wang, Jinbin,Zeng, Haijuan,et al. RPA-Cas12a-FS: A frontline nucleic acid rapid detection system for food safety based on CRISPR-Cas12a combined with recombinase polymerase amplification[J]. Food Chemistry,2021,334.
APA	Liu, Hua.,Wang, Jinbin.,Zeng, Haijuan.,Liu, Xiaofeng.,Jiang, Wei.,...&Tang, Xueming.(2021).RPA-Cas12a-FS: A frontline nucleic acid rapid detection system for food safety based on CRISPR-Cas12a combined with recombinase polymerase amplification.Food Chemistry,334.
MLA	Liu, Hua,et al."RPA-Cas12a-FS: A frontline nucleic acid rapid detection system for food safety based on CRISPR-Cas12a combined with recombinase polymerase amplification".Food Chemistry 334(2021).