Immobilized penicillin G acylase with enhanced activity and stability using glutaraldehyde-modified polydopamine-coated Fe3O4 nanoparticles

doi:10.1002/bab.2138

	Immobilized penicillin G acylase with enhanced activity and stability using glutaraldehyde-modified polydopamine-coated Fe3O4 nanoparticles
	Zhang, Boyuan 1,2; Zhou, Yongshan 1,2; Liu, Chunli 1,2; Abdelrahman Mohammed, Monier Alhadi 1,2; Chen, Zhangjun 1,2; Chen, Zhenbin 1,2
刊名	BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
	2021
卷号	69 期号:2 页码:629-641
关键词	Fe3O4 nanoparticles glutaraldehyde immobilization penicillin G acylase polydopamine
ISSN号	0885-4513
DOI	10.1002/bab.2138
英文摘要	In this work, Fe3O4 nanoparticles (NPs) were coated with polydopamine (PDA) to structure Fe3O4@PDA NPs by the spontaneous oxygen-mediated self-polymerization of dopamine (DA) in an aqueous solution of pH = 8.5. The fabricated Fe3O4@PDA NPs were grafted by glutaraldehyde to realize the immobilization of penicillin G acylase (PGA) under mild conditions. The carriers of each stage were characterized and investigated by transmission electron microscopy, X-ray diffraction, Fourier transform infrared, and vibrating sample magnetometry. To improve the catalytic activity and stability of immobilized PGA, the immobilization conditions were investigated and optimized. Under the optimal immobilization conditions, the enzyme loading capacity, enzyme activity, and enzyme activity recovery of immobilized PGA were 114 mg/g, 26,308 U/g, and 78.5%, respectively. In addition, the immobilized PGA presented better temperature and pH stability compared with free PGA. The reusability study ensured that the immobilized PGA showed an excellent repeating application performance. In particular, the recovery rate of immobilized PGA could reach 94.8% and immobilized PGA could retain 73.0% of its original activity after 12 cycles, indicating that the immobilized PGA exhibited a high operation stability and broad application potential in the biocatalysis field.
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
语种	英语
出版者	WILEY
WOS记录号	WOS:000629271300001
内容类型	期刊论文
源URL	[http://ir.lut.edu.cn/handle/2XXMBERH/148319]
专题	材料科学与工程学院
作者单位	1.Lanzhou Univ Technol, State Key Lab Adv Progressing & Recycling Nonferr, Lanzhou, Peoples R China 2.Lanzhou Univ Technol, Sch Mat Sci & Engn, Lanzhou, Peoples R China;
推荐引用方式 GB/T 7714	Zhang, Boyuan,Zhou, Yongshan,Liu, Chunli,et al. Immobilized penicillin G acylase with enhanced activity and stability using glutaraldehyde-modified polydopamine-coated Fe3O4 nanoparticles[J]. BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,2021,69(2):629-641.
APA	Zhang, Boyuan,Zhou, Yongshan,Liu, Chunli,Abdelrahman Mohammed, Monier Alhadi,Chen, Zhangjun,&Chen, Zhenbin.(2021).Immobilized penicillin G acylase with enhanced activity and stability using glutaraldehyde-modified polydopamine-coated Fe3O4 nanoparticles.BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,69(2),629-641.
MLA	Zhang, Boyuan,et al."Immobilized penicillin G acylase with enhanced activity and stability using glutaraldehyde-modified polydopamine-coated Fe3O4 nanoparticles".BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY 69.2(2021):629-641.